Signaling adaptor protein Crk is indispensable for malignant feature of glioblastoma cell line KMG4

Wang, Lei; Tabu, Kouichi; Kimura, Taichi; Tsuda, Masumi; Linghu, Hua; Tanino, Mishie; Kaneko, Sadao; Nishihara, Hiroshi; Tanaka, Shinya

Signaling adaptor protein Crk is indispensable for malignant feature of glioblastoma cell line KMG4

Wang, Lei; Tabu, Kouichi; Kimura, Taichi et al.

Biochemical and Biophysical Research Communications, 2007, 362(4), 976-981

Permalink : https://hdl.handle.net/2115/30182

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/30182
タイトル	Signaling adaptor protein Crk is indispensable for malignant feature of glioblastoma cell line KMG4
著者	Wang, Lei ; Tabu, Kouichi ; Kimura, Taichi ; Tsuda, Masumi ; Linghu, Hua ; Tanino, Mishie ; Kaneko, Sadao ; Nishihara, Hiroshi ; Tanaka, Shinya
著者名 Wang, Lei
著者名 Tabu, Kouichi
著者名 Kimura, Taichi
著者名 Tsuda, Masumi
著者名 Linghu, Hua
著者名 Tanino, Mishie
著者名 Kaneko, Sadao
著者名 Nishihara, Hiroshi
e-Rad_Researcher 70261287 著者名 Tanaka, Shinya
言語	英語
キーワード	Crk
	Adaptor
	Signaling
	Brain
	Glioblastoma
	Cancer
	SH2
	Invasion
	Adhesion
	Motility
発行日	2007-11-03
出版者	Elsevier Inc.
収録物名	Biochemical and Biophysical Research Communications
巻(号)	362(4)
ページ	976-981
抄録	Signaling adaptor protein Crk has been shown to be involved in pathogenesis of human cancers including brain tumor where Crk was reported to be overexpressed. In this study, we addressed whether Crk is indispensable for malignant phenotype of brain tumor. In 20 surgical specimens of glioma, mRNA of both CrkI and CrkII was found to be elevated in malignant tumor. To define a precise role of Crk, we have established Crk-knockdown cell lines of glioblastoma KMG4 by siRNA, and early phase of cell adhesion to laminin was found to be suppressed. Wound healing assay revealed the decreased cell motility in Crk knockdown cells, and suppression of both anchorage-dependent and -independent growth were demonstrated in these cells. Furthermore, in vivo tumor forming potential was also markedly suppressed. These results suggest that Crk is required for early attachment to laminin, cell motility, and growth of glioblastoma cell line KMG4.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0006-291X
EISSN	1090-2104
関連情報	PMID 17825249
関連情報	URI http://www.sciencedirect.com/science/journal/0006291X
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.bbrc.2007.08.106