Isolation of Individual Egg Cells and Zygotes in Alstroemeria Followed by Manual Selection with a Microcapillary-connected Micropump

HOSHINO, Yoichiro; MURATA, Naho; SHINODA, Koichi

Isolation of Individual Egg Cells and Zygotes in Alstroemeria Followed by Manual Selection with a Microcapillary-connected Micropump

HOSHINO, Yoichiro; MURATA, Naho; SHINODA, Koichi

Annals of Botany, 2006, 97(6), 1139-1144

Permalink : https://hdl.handle.net/2115/30230

このアイテムのアクセス数:1,286件（2026-08-10 05:41 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
AOB97-6	pdf	603 KB	1,475

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/30230
タイトル	Isolation of Individual Egg Cells and Zygotes in Alstroemeria Followed by Manual Selection with a Microcapillary-connected Micropump
著者	HOSHINO, Yoichiro ; MURATA, Naho ; SHINODA, Koichi
e-Rad_Researcher 50301875 著者名 HOSHINO, Yoichiro
著者名 MURATA, Naho
著者名 SHINODA, Koichi
言語	英語
キーワード	Alstroemeria aurea
	egg cell
	enzyme treatment
	microdissection
	zygote
発行日	2006-06
出版者	Oxford University Press
収録物名	Annals of Botany
巻(号)	97(6)
ページ	1139-1144
抄録	Aims: To develop a procedure for isolating living egg cells and zygotes from Alstroemeria ovules. Scope: We attempted to isolate egg cells and zygotes from the ovules of Alstroemeria aurea. The ovules were histologically observed using a clearing procedure which revealed the localization and sizes of the embryo sacs and egg apparatus within the ovules. For the isolation of egg cells, ovules were cut into sections with a surgical blade and treated with an enzyme solution. Subsequently, these ovule sections were dissected using a glass needle under an inverted microscope. Egg cells successfully isolated by this procedure were collected using microcapillaries connected to a micropump. For zygote isolation, ovules were excised from ovaries 24 hours after self-pollination. By treating excised ovules with an enzyme solution and subsequently dissecting them using a glass needle, zygotes were successfully isolated from the ovules and collected with a microcapillary. The isolated zygotes were associated with pollen tubes and one of the synergids. Egg cells and zygotes were viable for up to 2 h following isolation, as determined by fluorescein diacetate staining. Conclusions: The procedures for isolating egg cells and zygotes in Alstroemeria were established, and each egg cell and zygote was captured with a microcapillary.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Annals of Botany following peer review. The definitive publisher-authenticated version, YOICHIRO HOSHINO, NAHO MURATA and KOICHI SHINODA, Isolation of Individual Egg Cells and Zygotes in Alstroemeria Followed by Manual Selection with a Microcapillary-connected Micropump, Annals of Botany, 97(6), Jun 2006, pp.1139-1144 is available online at: http://aob.oxfordjournals.org/cgi/content/abstract/97/6/1139
PISSN	0305-7364
EISSN	1095-8290
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1093/aob/mcl072