RNA interference induced by siRNAs modified with 4'-thioribonucleosides in cultured mammalian cells

Hoshika, Shuichi; Minakawa, Noriaki; Kamiya, Hiroyuki; Harashima, Hideyoshi; Matsuda, Akira

RNA interference induced by siRNAs modified with 4'-thioribonucleosides in cultured mammalian cells

Hoshika, Shuichi; Minakawa, Noriaki; Kamiya, Hiroyuki et al.

FEBS Letters, 2005, 579(14), 3115-3118

Permalink : https://hdl.handle.net/2115/30353

このアイテムのアクセス数:1,516件（2026-08-25 00:12 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
FEBS-05-0515-revice	pdf	64.6 KB	1,607

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/30353
タイトル	RNA interference induced by siRNAs modified with 4'-thioribonucleosides in cultured mammalian cells
その他のタイトル	RNA interference (RNAi) induced by siRNAs modified with 4'-thioribonucleosides incultured mammalian cells
著者	Hoshika, Shuichi ; Minakawa, Noriaki ; Kamiya, Hiroyuki ; Harashima, Hideyoshi ; Matsuda, Akira
著者名 Hoshika, Shuichi
著者名 Minakawa, Noriaki
著者名 Kamiya, Hiroyuki
e-Rad_Researcher 00183567 著者名 Harashima, Hideyoshi
e-Rad_Researcher 90157313 著者名 Matsuda, Akira
言語	英語
キーワード	RNA interference (RNAi)
	Short interfering RNA (siRNA)
	Chemical modification
	4'-thioribonucleoside
	4'-thioRNA
発行日	2005-06-06
出版者	Elsevier B.V.
収録物名	FEBS Letters
巻(号)	579(14)
ページ	3115-3118
抄録	Short interfering RNAs (siRNAs) variously modified with 4'-thioribonucleosides against the Photinus luciferase gene were tested for their induction of the RNA interference (RNAi) activity in cultured NIH/3T3 cells. Results indicated that modifications at the sense-strand were well tolerated for RNAi activity except for full modification with 4'-thioribonucleosides. However, the activity of siRNAs modified at the antisense-strand was dependent on the position and the number of modifications with 4'-thioribonucleosides. Since modifications of siRNAs with 4'-thioribonucleosides were well tolerated in RNAi activity compared with that of 2'-O-methyl nucleosides, 4'-thioribonucleosides might be potentially useful in the development of novel and effective chemically modified siRNAs.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0014-5793
関連情報	PMID 15919084
関連情報	URI http://www.sciencedirect.com/science/journal/00145793
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.febslet.2005.04.073