open access

https://hdl.handle.net/2115/32751

Effect of honeycomb film on protein adsorption, cell adhesion and proliferation

英語

Self-organization

2006-08-15

Elsevier Science

Colloids and Surfaces. A, Physicochemical and Engineering Aspects

284-285

548-551

This article describes novel methods for controlling of cell adhesion by using micro porous polymer films. Recently we found the highly ordered micro porous films were formed when poly(ε-caprolactone)(PCL) solution was cast on substrates at high atmospheric humidity. The micro porous film has regular honeycomb morphology with a size of 5 μm per cell (honeycomb film). Endothelial cells grew rapidly on the honeycomb film. After 24 h cell culture, the cell number on honeycomb films was lager than that on PCL flat films. In order to elucidate the effect of honeycomb films as a scaffold for cell culture, the adsorbed proteins on honeycomb films under cell culture condition were observed. After conditioning of the honeycomb film and the flat film in DMEM containing 10 % foetal bovine serum (FBS) for 72 h at 37 ℃ in 5% CO2 atmosphere, the adsorbed fibronectin-FITC and albumin-Texasred on the honeycomb films was observed by using confocal laser scanning microscope (CLSM). The observation revealed that fibronectin showed site-selective adsorption behavior on the honeycomb film. Albumin adsorbed on the honeycomb film non site-selectively, while fibronectin mainly adsorbed on inside of honeycomb pores. On the flat film, fibronectin was hardly observed. Since the honeycomb film accelerates the adsorption of fibronectin which is a typical protein as a cell adhesion molecule, the film could be a scaffold with excellent cell adhesion properties.

学術雑誌論文

Accepted Manuscript

0927-7757