Radiation-induced apoptosis of tumor cells is facilitated by inhibition of the interaction between Survivin and Smac/DIABLO

Ogura, Aki; Watanabe, Yasuko; Iizuka, Daisuke; Yasui, Hironobu; Amitani, Makoto; Kobayashi, Saori; Kuwabara, Mikinori; Inanami, Osamu

Radiation-induced apoptosis of tumor cells is facilitated by inhibition of the interaction between Survivin and Smac/DIABLO

Ogura, Aki; Watanabe, Yasuko; Iizuka, Daisuke et al.

Cancer Letters, 2008, 259(1), 71-81

Permalink : https://hdl.handle.net/2115/32876

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アクセス権	open access
URI	https://hdl.handle.net/2115/32876
タイトル	Radiation-induced apoptosis of tumor cells is facilitated by inhibition of the interaction between Survivin and Smac/DIABLO
著者	Ogura, Aki ; Watanabe, Yasuko ; Iizuka, Daisuke ; Yasui, Hironobu ; Amitani, Makoto ; Kobayashi, Saori ; Kuwabara, Mikinori ; Inanami, Osamu
著者名 Ogura, Aki
著者名 Watanabe, Yasuko
著者名 Iizuka, Daisuke
e-Rad_Researcher 10570228 著者名 Yasui, Hironobu
著者名 Amitani, Makoto
著者名 Kobayashi, Saori
著者名 Kuwabara, Mikinori
e-Rad_Researcher 10193559 著者名 Inanami, Osamu
言語	英語
キーワード	Survivin
	radiation
	apoptosis
	dominant negative mutant
	adenoviral vector
発行日	2008-01-18
出版者	Elsevier
収録物名	Cancer Letters
巻(号)	259(1)
ページ	71-81
抄録	To investigate the mechanism of radioresistance of solid tumor cells, we created two expression vectors encoding Survivin mutants, T34A and D53A. When T34A and D53A were overexpressed in NIH3T3, A549 and HeLa cells, radiation-induced apoptosis was significantly enhanced. Furthermore, we examined the binding capability of Survivin with Smac/DIABLO in the cells that overexpressed these mutants. Coimmunoprecipitation analysis revealed that mutant form of Survivin, D53A and T34A could bind to Smac/DIABLO, but with much less affinity compared to the authentic form. These results suggest that radiation-induced apoptosis of tumor cells is increased by inhibition of the interaction between Survivin and Smac/DIABLO through overexpression of T34A and D53A.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0304-3835
関連情報	PMID 17967504
関連情報	URI http://www.sciencedirect.com/science/journal/03043835
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.canlet.2007.09.017