Enhancement of Cell Death by TNF α-related Apoptosis-inducing Ligand (TRAIL) in Human Lung Carcinoma A549 Cells Exposed to X Rays under Hypoxia

Takahashi, Momoko; Inanami, Osamu; Kubota, Nobuo et al.

Journal of Radiation Research, 2007, 48(6), 461-468

Permalink : https://hdl.handle.net/2115/32929

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アクセス権
open access
URI
https://hdl.handle.net/2115/32929
タイトル
Enhancement of Cell Death by TNF α-related Apoptosis-inducing Ligand (TRAIL) in Human Lung Carcinoma A549 Cells Exposed to X Rays under Hypoxia
著者
Takahashi, Momoko ; Inanami, Osamu ; Kubota, Nobuo ; Tsujitani, Michihiko ; Yasui, Hironobu ; Ogura, Aki ; Kuwabara, Mikinori
著者名
Takahashi, Momoko
e-Rad_Researcher
10193559
著者名
Inanami, Osamu
著者名
Kubota, Nobuo
著者名
Tsujitani, Michihiko
e-Rad_Researcher
10570228
著者名
Yasui, Hironobu
著者名
Ogura, Aki
著者名
Kuwabara, Mikinori
言語
英語
キーワード
TRAIL
radiation
caspase
hypoxia
発行日
2007-11
出版者
日本放射線影響学会
収録物名
Journal of Radiation Research
巻(号)
48(6)
ページ
461-468
抄録
Our previous study showed that ionizing radiation induced the expression of death receptor DR5 on the cell surface in tumor cell lines and that the death receptor of the TNF alpha-related apoptosis-inducing ligand TRAIL enhanced the apoptotic pathway (Hamasu et al., (2005) Journal of Radiation Research, 46:103-110). The present experiments were performed to examine whether treatment with TRAIL enhanced the cell killing in tumor cells exposed to ionizing radiation under hypoxia, since the presence of radioresistant cells in hypoxic regions of solid tumors is a serious problem in radiation therapy for tumors. When human lung carcinoma A549 cells were irradiated under normoxia and hypoxia, respectively, radiation-induced enhancement of expression of DR5 was observed under both conditions. Incubation in the presence of TRAIL enhanced the caspase-dependent and chymotrypsin-like-protease-dependent apoptotic cell death in A549 cells exposed to X rays. Furthermore, it was shown that treatment with TRAIL enhanced apoptotic cell death and loss of clonogenic ability in A549 cells exposed to X rays not only under normoxia but also under hypoxia, suggesting that combination treatment with TRAIL and X irradiation is effective for hypoxic tumor cells.
資源タイプ
学術雑誌論文
出版タイプ
Version of Record
PISSN
0449-3060
EISSN
1349-9157
関連情報 (isIdenticalTo)
出版社版DOI
https://doi.org/10.1269/jrr.07028