open access

https://hdl.handle.net/2115/32949

Genomic Structure and Expression of the Sea Urchin Soluble Guanylyl Cyclase β Subunit Gene

英語

Hemicentrotus pulcherrimus

2001-08

日本動物学会

Zoological Science

18(6)

811-817

We obtained the full-length cDNA and genomic DNA clones of HpGCS-β1 encoding theβsubunit of soluble guanylyl cyclase (soluble GC), which is expressed in the testis and ovary of the sea urchin Hemicentrotus pulcherrimus. Reverse transcription-polymerase chain reaction analysis demonstrated that the HpGCS-β1 transcript was detected in unfertilized eggs as well as in the testis and ovary. The open reading frame predicted a protein of 604 amino acids with a putative cyclase catalytic domain conserved in membrane GCs and adenylyl cyclases. 105His, 78Cys, and 122Cys, important amino acids for heme-binding in theβ1 subunit of soluble GCs in vertebrates, were conserved in the corresponding positions of HpGCS-β1. The HpGCS-β1 gene consisted of 8 exons and had a span of 26 kbp. A comparison of genomic structure of the HpGCS-β1 gene with that of the soluble GCβ1 subunit gene (OlGCS-β1) of the medaka fish indicated that exon 4 in the HpGCS-β1 gene corresponds to exons 4 to 9 in the OlGCS-β1 gene.

学術雑誌論文

Version of Record

(c) 日本動物学会 / 本文献の公開は著者の意思に基づくものである

0289-0003