Genomic Structure and Expression of the Soluble Guanylyl Cyclase α2 Subunit Gene in the Medaka Fish Oryzias latipes

Yao, Yuko; Yamamoto, Takehiro; Tsutsumi, Makiko; Matsuda, Masaru; Hori, Hiroshi; Naruse, Kiyoshi; Mitani, Hiroshi; Shima, Akihiro; Asakawa, Shuichi; Shimizu, Nobuyoshi; Suzuki, Norio

Genomic Structure and Expression of the Soluble Guanylyl Cyclase α2 Subunit Gene in the Medaka Fish Oryzias latipes

Yao, Yuko; Yamamoto, Takehiro; Tsutsumi, Makiko et al.

Zoological Science, 2003, 20(10), 1293-1304

Permalink : https://hdl.handle.net/2115/32965

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アクセス権	open access
URI	https://hdl.handle.net/2115/32965
タイトル	Genomic Structure and Expression of the Soluble Guanylyl Cyclase α2 Subunit Gene in the Medaka Fish Oryzias latipes
著者	Yao, Yuko ; Yamamoto, Takehiro ; Tsutsumi, Makiko ; Matsuda, Masaru ; Hori, Hiroshi ; Naruse, Kiyoshi ; Mitani, Hiroshi ; Shima, Akihiro ; Asakawa, Shuichi ; Shimizu, Nobuyoshi ; Suzuki, Norio
著者名 Yao, Yuko
著者名 Yamamoto, Takehiro
著者名 Tsutsumi, Makiko
著者名 Matsuda, Masaru
著者名 Hori, Hiroshi
著者名 Naruse, Kiyoshi
著者名 Mitani, Hiroshi
著者名 Shima, Akihiro
著者名 Asakawa, Shuichi
著者名 Shimizu, Nobuyoshi
著者名 Suzuki, Norio
言語	英語
キーワード	soluble guanylyl cyclase
	cGMP
	medaka fish
	gene expression
	exon/intron organization
発行日	2003-10
出版者	日本動物学会
収録物名	Zoological Science
巻(号)	20(10)
ページ	1293-1304
抄録	A cDNA clone encoding the soluble guanylyl cyclase α2 subunit was isolated from medaka fish (Oryzias latipes) and designated as OIGCS-α2. The OIGCS-α2 cDNA was 3192 bp in length and the open reading frame (ORF) encodes a protein of 805 amino acids. The deduced amino acid sequence has high similarity to that of the mammalianα2 subunit gene except for the N-terminal regulatory domain. The C-terminal 5 amino acids, "RETSL", which have been reported to interact with the post synaptic density protein (PSD)-95 were conserved. An RNase protection assay with adult fish organs showed that O/GCS-α2 was expressed mainly in the brain and testis. The complete nucleotide sequence (about 41 kbp) of the OIGCS-α2 genomic DNA clone isolated from a medaka fish BAC library indicated that the OIGCS-α2 gene consisted of 9 exons and 8 introns. The 5'-flanking region and larger introns, such as introns 1, 4, and 7, contained the several fragments conserved in the nucleotide sequences of Rex6 (non-long terminal repeat retrotransposon), MHC class I genomic region, and OIGC1, the medaka fish homolog of the mammalian guanylyl cyclase B gene. Linkage analysis on the medaka fish chromosome demonstrated that the OIGCS-α2 gene was mapped to LG13; this mapping position was different from those for the OIGCS-α1 and OIGCS-β1 genes (LG1).
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	(c) 日本動物学会 / 本文献の公開は著者の意思に基づくものである
PISSN	0289-0003
関連情報	URI http://www.bioone.org/perlserv/?request=get-archive&issn=0289-0003