open access

https://hdl.handle.net/2115/33897

An RNA biding protein, Y14 interacts with and modulates STAT3 activation

Interactions between STAT3 and Y14

英語

signal transduction

2008-08-01

Academic Press

Biochemical and Biophysical Research Communications

372(3)

475-479

Signal transducer and activator of transcription 3 (STAT3), which mediates biological actions in many physiological processes, is activated by cytokines and growth factors via specific tyrosine phosphorylation, dimerization and nuclear translocation. To clarify the molecular mechanisms underlying the regulation of STAT3 activation, we performed yeast two-hybrid screening. We identified Y14, an RNA-binding protein, as a novel STAT3-binding partner. Y14 bound to STAT3 through the C-terminal region of STAT3 in vivo. Importantly, small-interfering RNA-mediated reduction of endogenous Y14 expression decreased IL-6-induced tyrosine-phosphorylation, nuclear accumulation and DNA binding activity of STAT3, as well as IL-6/STAT3-dependent gene expression. These results indicate that Y14 interacts with STAT3 and regulates the transcriptional activation of STAT3 by influencing the tyrosine-phosphorylation of STAT3.

学術雑誌論文

Accepted Manuscript

0006-291X

1090-2104

AA00564395