X Irradiation Combined with TNF α-related Apoptosis-inducing Ligand (TRAIL) Reduces Hypoxic Regions of Human Gastric Adenocarcinoma Xenografts in SCID Mice

TAKAHASHI, Momoko; YASUI, Hironobu; OGURA, Aki; ASANUMA, Taketoshi; KUBOTA, Nobuo; TSUJITANI, Michihiko; KUWABARA, Mikinori; INANAMI, Osamu

X Irradiation Combined with TNF α-related Apoptosis-inducing Ligand (TRAIL) Reduces Hypoxic Regions of Human Gastric Adenocarcinoma Xenografts in SCID Mice

TAKAHASHI, Momoko; YASUI, Hironobu; OGURA, Aki et al.

Journal of Radiation Research, 2008, 49(2), 153-161

Permalink : https://hdl.handle.net/2115/33951

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アクセス権	open access
URI	https://hdl.handle.net/2115/33951
タイトル	X Irradiation Combined with TNF α-related Apoptosis-inducing Ligand (TRAIL) Reduces Hypoxic Regions of Human Gastric Adenocarcinoma Xenografts in SCID Mice
著者	TAKAHASHI, Momoko ; YASUI, Hironobu ; OGURA, Aki ; ASANUMA, Taketoshi ; KUBOTA, Nobuo ; TSUJITANI, Michihiko ; KUWABARA, Mikinori ; INANAMI, Osamu
著者名 TAKAHASHI, Momoko
著者名 YASUI, Hironobu
著者名 OGURA, Aki
著者名 ASANUMA, Taketoshi
著者名 KUBOTA, Nobuo
著者名 TSUJITANI, Michihiko
著者名 KUWABARA, Mikinori
e-Rad_Researcher 10193559 著者名 INANAMI, Osamu
言語	英語
キーワード	Radiation
	Solid tumor
	TRAIL
	Hypoxia
	Xenograft
発行日	2008-03-26
出版者	Japan Radiation Research Society
収録物名	Journal of Radiation Research
巻(号)	49(2)
ページ	153-161
抄録	Our previous study showed that X irradiation induced the expression of death receptor DR5 on the cell surface in tumor cell lines under not only normoxia but also hypoxia. X irradiation combined with TNF α-related apoptosis-inducing ligand (TRAIL), which is the ligand of DR5, induced apoptosis in vitro (Takahashi et al., (2007) Journal of Radiation Research, 48: 461-468). In this report, we examined the in vivo antitumor efficacy of X irradiation combined with TRAIL treatment in tumor xenograft models derived from human gastric adenocarcinoma MKN45 and MKN28 cells in SCID mice. X irradiation combined with TRAIL synergistically suppressed the tumor growth rates in the xenograft models derived from MKN45 and MKN28 cells, which have wild type Tp53 and mutated Tp53, respectively, indicating that the antitumor effects occurred in a Tp53-independent manner. Histological analysis showed that the combination of X irradiation and TRAIL induced caspase-3-dependent apoptotic cell death. Moreover, the immunohistochemical detection of hypoxic regions using the hypoxic marker pimonidazole revealed that caspase-3-dependent apoptosis occurred in the hypoxic regions in the tumors. These results indicated that X irradiation combined with TRAIL may be a useful treatment to reduce tumor growth in not only normoxic but also hypoxic regions.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	0449-3060
EISSN	1349-9157
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1269/jrr.07082