Morphological investigations of disaccharide molecules for growth inhibition of ice crystals

Uchida, Tsutomu; Nagayama, Masafumi; Shibayama, Tamaki et al.

Journal of Crystal Growth, 2007, 299(1), 125-135

Permalink : https://hdl.handle.net/2115/36102

このアイテムのアクセス数:1,767件（2026-08-16 08:05 集計）

閲覧可能ファイル 

ファイル フォーマット サイズ 閲覧回数 説明
uchida-5 pdf 4.29 MB 2,136

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権
open access
URI
https://hdl.handle.net/2115/36102
タイトル
Morphological investigations of disaccharide molecules for growth inhibition of ice crystals
著者
Uchida, Tsutomu ; Nagayama, Masafumi ; Shibayama, Tamaki ; Gohara, Kazutoshi
e-Rad_Researcher
70356575
著者名
Uchida, Tsutomu
e-Rad_Researcher
70374585
著者名
Nagayama, Masafumi
著者名
Shibayama, Tamaki
e-Rad_Researcher
40153746
著者名
Gohara, Kazutoshi
言語
英語
キーワード
A1. Nanostructures
A1. Solubilities
A2. Growth from solutions
B1. Biological substances
B1. Sucrose
PACS = 81.10.Dn
発行日
2007-02-01
出版者
Elsevie
収録物名
Journal of Crystal Growth
巻(号)
299(1)
ページ
125-135
抄録
Freezing of solutions including disaccharides (trehalose, sucrose, and maltose) has been investigated by microscopic observations of freeze-fractured replicas using FE-TEM. Three typical features were observed: the smooth surface considered as the ice crystal, fine particles as the precipitated disaccharide molecules, and remaining part as the glass state of the solution. The expanded observations of fine-particle and its distribution investigations suggested that it was larger than 10 nm in size and averaged approximately 20-30 nm in diameter. The smallest particle was estimated to include several hundred disaccharide molecules. Based on systematic observations of trehalose solutions regarding concentrations and freezing rates, we concluded that ice crystal growth was inhibited by trehalose molecules. Since the ice crystal size reduced exponentially with increase in trehalose concentration, we could control ice crystal size formed in the frozen material. The growth inhibition of ice crystals with trehalose resulted both from a reduction in the free water in the solution due to a significant hydration effect and from an enhancement of nucleation of the ice crystals. It was confirmed that trehalose was more effective than the other disaccharide solutions examined for inhibiting the growth of ice crystals.
資源タイプ
学術雑誌論文
出版タイプ
Accepted Manuscript
PISSN
0022-0248
関連情報 (isVersionOf)
出版社版DOI
https://doi.org/10.1016/j.jcrysgro.2006.10.261