open access

https://hdl.handle.net/2115/36650

Detection of changes in the nuclear phase and evaluation of male germ units by flow cytometry during in vitro pollen tube growth in Alstroemeria aurea

英語

sperm cell

2009-03

Botanical Society of Japan

Journal of Plant Research

122(2)

225-234

This study aimed to analyze male gamete behavior from mature pollen to pollen tube growth in the bicellular pollen species Alstroemeria aurea. For mature pollen, pollen protoplasts were examined using flow cytometry. The protoplasts showed two peaks of DNA content at 1C and 1.90C. Flow cytometry of pollen tubes at different developmental stages that were cultured in vitro revealed changes in the nuclear phase at 9 h and 18 h after culture. Sperm cell formation occurred at 6–9 h after culture, indicating that the first change was due to the division of the generative cells into sperm cells. After sperm cell formation, the number of vegetative nucleus associations with sperm cell showed a tendency to increase. The association was suggested as male germ unit. When sperm cells, vegetative nuclei, and partial male germ units were separately collected from pollen tubes cultured for 18 h and analyzed using a flow cytometer, the sperm cells and vegetative nuclei contained 1C DNA, while the DNA content of partial male germ units was counted as 2C. Therefore, the second change in the nuclear phase, which resulted in an increase in 2C nuclei, was possibly related to the formation of male germ units.

学術雑誌論文

Accepted Manuscript

The original publication is available at www.springerllink.com.

0918-9440

1618-0860

AA10900218