Detection of antibody against Aeromonas salmonicida in the serum of salmonid fish by the enzyme linked immunosorbent assay

Yoshimizu, Mamoru; Direkbusarakom, Sataporn; Nomura, Tetsuichi et al.

魚病研究, 1992, 27(2), 73-82

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アクセス権
open access
URI
https://hdl.handle.net/2115/38337
タイトル
Detection of antibody against Aeromonas salmonicida in the serum of salmonid fish by the enzyme linked immunosorbent assay
著者
Yoshimizu, Mamoru ; Direkbusarakom, Sataporn ; Nomura, Tetsuichi ; Ezura, Yoshio ; Kimura, Takahisa
e-Rad_Researcher
40122915
著者名
Yoshimizu, Mamoru
著者名
Direkbusarakom, Sataporn
著者名
Nomura, Tetsuichi
著者名
Ezura, Yoshio
著者名
Kimura, Takahisa
言語
英語
発行日
1992-06
出版者
日本魚病学会
Japanese Society of Fish Pathology
収録物名
魚病研究
Fish pathology
巻(号)
27(2)
ページ
73-82
抄録
Enzyme linked immunosorbent assay (ELISA) using the anti-masu Ig rabbit serum was applied to detect the antibody against Aeromonas salmonicida in the sera or salmonid fish. This ELISA method could detect the antibody in sera of masu (Oncorhynchus masou), chum (O. keta) and coho salmon (O. kisutch) immunized by A. salmonicida and could be applied to detect the antibody against A. salmonicida in sera of matured masu, pink (O. gorbuscha), chum, and kokanee salmon (O. nerka). In 1989, 89 masu and 250 chum salmon, and 59 kokanee were examined to detect antibody against A. salmonicida by ELISA and agglutination test. The number of the fish showing the positive reaction by ELISA, agglutination test and isolation of A. salmonicida were 262, 48, and 33, respectively. In 1990, 200 masu and kokanee salmon fry released from hatchery to river were tested and antibody against A. salmonicida was detected from 111 fish by ELISA, and the incidence of the fish showing the positive reaction was ranged from 4 to 90%.
資源タイプ
学術雑誌論文
出版タイプ
Version of Record
PISSN
0388-788X
NCID
AN00063165