open access

https://hdl.handle.net/2115/38379

Limited Availability of PCR for the Detection of Renibacterium salmoninarum, the Causative Agent of Bacterial Kidney Disease

日本語

IFAT

2008-03

Japanese Society of Fish Pathology

Fish Pathology

43(1)

29-33

Detection limit of PCR was investigated for Renibacterium salmoninarum, the causative agent of bacterial kidney disease, with a logarithmic growth phase. When genomic DNAs from serially diluted bacterial cells were subjected to PCR, the minimum concentration by PCR-detection with 30, 35 and 40 cycles of amplification were all 10^5 cells/mL, and the sensitivity was not improved by treatments of the bacteria with lysozyme, achromopeptidase and/or SDS. The extraction rate of genomic DNAs from the bacterial cells was approximately 3%. It was confirmed that PCR-detection limit for R. salmoninarum cells mixed with fish kidney tissues was significantly lower than that for the bacteria suspended in the medium. Moreover, there was no significant difference in the detection limit between immunofluorescence antibody technique (IFAT) and PCR. These results suggest that PCR detection should be followed by other methods such as IFAT.

学術雑誌論文

Version of Record

0388-788X

AN00063165