The protein content of an adaptor protein, STAP-2 is controlled by E3 ubiquitin ligase Cbl

Sekine, Yuichi; Yamamoto, Chikako; Ikeda, Osamu et al.

Biochemical and Biophysical Research Communications, 2009, 384(2), 187-192

Permalink : https://hdl.handle.net/2115/38461

このアイテムのアクセス数:1,317件（2026-08-14 07:44 集計）

閲覧可能ファイル 

ファイル フォーマット サイズ 閲覧回数 説明
matsuda pdf 767 KB 1,391

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権
open access
URI
https://hdl.handle.net/2115/38461
タイトル
The protein content of an adaptor protein, STAP-2 is controlled by E3 ubiquitin ligase Cbl
その他のタイトル
Cbl controls STAP-2 activity through protein degradation
著者
Sekine, Yuichi ; Yamamoto, Chikako ; Ikeda, Osamu ; Muromoto, Ryuta ; Nanbo, Asuka ; Oritani, Kenji ; Yoshimura, Akihiko ; Matsuda, Tadashi
著者名
Sekine, Yuichi
著者名
Yamamoto, Chikako
著者名
Ikeda, Osamu
著者名
Muromoto, Ryuta
著者名
Nanbo, Asuka
著者名
Oritani, Kenji
著者名
Yoshimura, Akihiko
e-Rad_Researcher
20212219
著者名
Matsuda, Tadashi
言語
英語
キーワード
STAP-2
Cbl
protein degradation
発行日
2009-06-26
出版者
Academic Press
収録物名
Biochemical and Biophysical Research Communications
巻(号)
384(2)
ページ
187-192
抄録
Signal transducing adaptor protein-2 (STAP-2) is a recently identified adaptor protein that contains pleckstrin and Src homology 2 (SH2)-like domains as well as a YXXQ motif in its C-terminal region. Our previous study in T cells demonstrated that STAP-2 influences FAK protein levels through recruitment of E3 ubiquitin ligase, Cbl, to FAK. In the present study, we found that Cbl directly controls the protein levels and activity of STAP-2. STAP-2 physically interacted with Cbl through its PH and SH2-like domains. Small-interfering RNA-mediated reduction of endogenous Cbl restored STAP-2 protein levels. In contrast, over-expression of Cbl induced STAP-2 degradation. Importantly, Cbl-mediated regulation of STAP-2 protein levels affected Brk/STAP-2-induced STAT3 activation. These results indicate that Cbl regulates STAP-2 protein levels and Brk/STAP-2-mediated STAT3 activation.
資源タイプ
学術雑誌論文
出版タイプ
Accepted Manuscript
PISSN
0006-291X
NCID
AA00564395
関連情報
PMID
19401194
関連情報 (isVersionOf)
出版社版DOI
https://doi.org/10.1016/j.bbrc.2009.04.109