Aeromonas salmonicida 自然凝集株の血清学的鑑別への特異抗体感作 staphylococci を用いた coagglutination test の応用

日本水産学会

日本水産学会誌

The majority of isolates of Aeromonas salmonicida, the causative organism of fish of furunculosis, from diseased fish are rough, that is auto-agglutinating. Therefore serological identification by the slide agglutination test may be difficult to perform. The application of a coagglutination test, for the serological identification of auto-agglutinate A. salmonicida, was studied using staphylococci specifically sensitized with an antibody against the bacterium. This proved to be a simple and rapid method for use in the laboratory and required no special apparatus. The procedure for this method is summarized as follows. 1. A. salmonicida, cultured in nutrient broth, is heated in boiling water for 30min. 2. The supernatant is collected after centrifugation at 4000rpm for 20min. This may be omitted if auto-agglutination is recognized at the bottom of the culture tube. 3. One drop of the supernatant and one drop of anti-A. salmonicida antibody sensitized staphylococci suspension are mixed on a glass slide and the slide is examined after 30min.

© 1984 公益社団法人日本水産学会