Incorporation of 8-hydroxyguanosine (8-oxo-7,8-dihydroguanosine) 5'-triphosphate by bacterial and human RNA polymerases

Kamiya, Hiroyuki; Suzuki, Akihiro; Yamaguchi, Yuki; Handa, Hiroshi; Harashima, Hideyoshi

Incorporation of 8-hydroxyguanosine (8-oxo-7,8-dihydroguanosine) 5'-triphosphate by bacterial and human RNA polymerases

Kamiya, Hiroyuki; Suzuki, Akihiro; Yamaguchi, Yuki et al.

Free Radical Biology and Medicine, 2009, 46(12), 1703-1707

Permalink : https://hdl.handle.net/2115/38630

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アクセス権	open access
URI	https://hdl.handle.net/2115/38630
タイトル	Incorporation of 8-hydroxyguanosine (8-oxo-7,8-dihydroguanosine) 5'-triphosphate by bacterial and human RNA polymerases
その他のタイトル	Incorporation of 8-hydroxyguanosine 5'-triphosphate (8-oxo-7,8-dihydroguanosine 5'-triphosphate) by bacterial and human RNA polymerases
著者	Kamiya, Hiroyuki ; Suzuki, Akihiro ; Yamaguchi, Yuki ; Handa, Hiroshi ; Harashima, Hideyoshi
著者名 Kamiya, Hiroyuki
著者名 Suzuki, Akihiro
著者名 Yamaguchi, Yuki
著者名 Handa, Hiroshi
e-Rad_Researcher 00183567 著者名 Harashima, Hideyoshi
言語	英語
キーワード	Oxidized ribonucleotide
	8-Hydroxyguanosine 5'-triphosphate
	RNA polymerase
	Transcription
	Free radicals
発行日	2009-06-15
出版者	Elsevier Inc.
収録物名	Free Radical Biology and Medicine
巻(号)	46(12)
ページ	1703-1707
抄録	Oxidized RNA precursors formed in the nucleotide pool may be incorporated into RNA. In this study, the incorporation of 8-hydroxyguanosine 5'-triphosphate (8-OH-GTP; 8-oxo-7,8-dihydroguanosine 5'-triphosphate) into RNA by Escherichia coli RNA polymerase was examined in vitro, using a primer RNA and a template DNA with defined sequences. 8-OH-GTP was incorporated opposite C and A in the template DNA. Surprisingly, 8-OH-GTP was quite efficiently incorporated by the bacterial RNA polymerase, in contrast to the incorporation of the 2'-deoxyribo-counterpart by DNA polymerases, as indicated by the kinetic parameters. The primer was further extended by the addition of a ribonucleotide complementary to the nucleobase adjacent to C or A (the nucleobase opposite which 8-OH-GTP was inserted). Thus, the incorporation of 8-OH-GTP did not completely inhibit further RNA chain elongation. 8-OH-GTP was also incorporated opposite C and A by human RNA polymerase II. These results suggest that 8-OH-GTP in the nucleotide pool call cause the formation of oxidized RNA and disturb the transmittance of genetic information.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0891-5849
関連情報	PMID 19362141
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.freeradbiomed.2009.04.005