Effects of carriers on transgene expression from plasmids containing a DNA sequence with high histone affinity

Kamiya, Hiroyuki; Fukunaga, Satoki; Ohyama, Takashi; Harashima, Hideyoshi

Effects of carriers on transgene expression from plasmids containing a DNA sequence with high histone affinity

Kamiya, Hiroyuki; Fukunaga, Satoki; Ohyama, Takashi et al.

International Journal of Pharmaceutics, 2009, 376(1-2), 99-103

Permalink : https://hdl.handle.net/2115/38635

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アクセス権	open access
URI	https://hdl.handle.net/2115/38635
タイトル	Effects of carriers on transgene expression from plasmids containing a DNA sequence with high histone affinity
著者	Kamiya, Hiroyuki ; Fukunaga, Satoki ; Ohyama, Takashi ; Harashima, Hideyoshi
著者名 Kamiya, Hiroyuki
著者名 Fukunaga, Satoki
著者名 Ohyama, Takashi
e-Rad_Researcher 00183567 著者名 Harashima, Hideyoshi
言語	英語
キーワード	left-handedly curved DNA
	histones
	plasmid DNA
	intranuclear disposition
	carriers
発行日	2009-07-06
出版者	Elsevier B.V.
収録物名	International Journal of Pharmaceutics
巻(号)	376(1-2)
ページ	99-103
抄録	The intranuclear disposition of plasmid DNA is highly important for transgene expression. The effects of a left-handedly curved sequence with high histone affinity on transgene expression were examined in COS-7 cells with two kinds of carriers (Lipofectamine Plus and TransIT-LT1). Three plasmids containing the curved sequence at different positions were transfected. The transgene expression was affected by the position of the left-handedly curved sequence, and the sequence at appropriate locations enhanced the expression from plasmid DNAs. However, the position effects on the expression differed from those obtained by electroporation of the same plasmid DNAs in a naked form. In addition, the degree of expression enhancement seemed to depend on the carriers. These results suggest that the left-handedly curved sequence with high histone affinity could increase the transgene expression from a plasmid delivered with carriers.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0378-5173
関連情報	PMID 19409470
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.ijpharm.2009.04.032