Molecular characterization and histochemical demonstration of salmon olfactory marker protein in the olfactory epithelium of lacustrine sockeye salmon (Oncorhynchus nerka)

Kudo, H.; Doi, Y.; Ueda, H.; Kaeriyama, M.

Molecular characterization and histochemical demonstration of salmon olfactory marker protein in the olfactory epithelium of lacustrine sockeye salmon (Oncorhynchus nerka)

Kudo, H.; Doi, Y.; Ueda, H. et al.

Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, 2009, 154(1), 142-150

Permalink : https://hdl.handle.net/2115/38841

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アクセス権	open access
URI	https://hdl.handle.net/2115/38841
タイトル	Molecular characterization and histochemical demonstration of salmon olfactory marker protein in the olfactory epithelium of lacustrine sockeye salmon (Oncorhynchus nerka)
著者	Kudo, H. ; Doi, Y. ; Ueda, H. ; Kaeriyama, M.
e-Rad_Researcher 40289575 著者名 Kudo, H.
著者名 Doi, Y.
e-Rad_Researcher 00160177 著者名 Ueda, H.
e-Rad_Researcher 80305937 著者名 Kaeriyama, M.
言語	英語
キーワード	olfactory marker protein
	olfactory receptor neuron
	cloning
	in situ hybridization
	immunocytochemistry
	Pacific salmon
	Teleostei
発行日	2009-09
出版者	Elsevier
収録物名	Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
巻(号)	154(1)
ページ	142-150
抄録	Despite the importance of olfactory receptor neurons (ORNs) for homing migration, the expression of olfactory marker protein (OMP) is not well understood in ORNs of Pacific salmon (Genus Oncorhynchus). In this study, salmon OMP was characterized in the olfactory epithelia of lacustrine sockeye salmon (O. nerka) by molecular biological and histochemical techniques. Two cDNAs encoding salmon OMP were isolated and sequenced. These cDNAs both contained a coding region encoding 173 amino acid residues, and the molecular mass of the two proteins was calculated to be 19,581.17 and 19,387.11 Da, respectively. Both amino acid sequences showed marked homology (90%). The protein and nucleotide sequencing demonstrates the existence of high-level homology between salmon OMPs and those of other teleosts. By in situ hybridization using a digoxigeninlabeled salmon OMP cRNA probe, signals for salmon OMP mRNA were observed preferentially in the perinuclear regions of the ORNs. By immunohistochemistry using a specific antibody to salmon OMP, OMP-immunoreactivities were noted in the cytosol of those neurons. The present study is the first to describe cDNA cloning of OMP in salmon olfactory epithelium, and indicate that OMP is a useful molecular marker for the detection of the ORNs in Pacific salmon.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	1095-6433
NCID	AA11183804
関連情報	PMID 19501666
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.cbpa.2009.05.123