open access

https://hdl.handle.net/2115/38894

Development of a 3D collagen scaffold coated with multiwalled carbon nanotubes

英語

cell culture

2009-08

Wiley

Journal of Biomedical Materials Research. Part B, Applied Biomaterials

90b(2)

629-634

Carbon nanotubes (CNTs) have attractive biochemical properties such as strong cell adhesion and protein absorption, which are very useful for a cell cultivation scaffold. In this study, we prepared a multiwalled carbon nanotube-coated collagen sponge (MWCNT-coated sponge) to improve the surface properties of the collagen sponge, and its cell culturing properties were examined. The suface of the collagen sponge was homogeneously coated with MWCNTs by dispersion. MC3T3-E1 cells were cultured on and inside the MWCNT-coated sponge. The DNA content on the MWCNT-coated sponge after 1 week of culture was significantly higher than on an uncoated collagen sponge (p<0.05). There was no significant difference between the estimated ALP activity normalized by DNA quantity on the MWCNT-coated sponge and that on the uncoated collagen sponge which is well known as one of the best scaffolds for cell cultivation. In addition, the MWCNT-coated surface shows strong cell adhesion. Therefore, the MWCNT-coated collagen sponge is expected to be a useful 3D scaffold for cell cultivation.

学術雑誌論文

Accepted Manuscript

This is the pre-peer reviewed version of the following article: Journal of Biomedical Materials Research, Part B, 90B(2), 2009, pp.629-634, which has been published in final form at http://dx.doi.org/10.1002/jbm.b.31327

1552-4973

1552-4981

AA11881538