open access

https://hdl.handle.net/2115/39116

Dynamic metabolic profiling using in vivo stable isotope labeling

日本語

2009-03-31

Institute of Low Temperature Science, Hokkaido University

Low Temperature Science

67

169-174

3章 単離・精製・活性測定 1. 代謝産物量の定量 b

Metabolic profiling technique has enabled to analyze the level of various metabolites including minor intermediates by using MS or NMR simultaneously, but the information available from the technique was snapshot in vivo at the time of sampling metabolites. In the dynamic steady state, metabolites are replaced with newly-synthesized compounds at a constant rate and the total amount of metabolites remains unchanged. Therefore, in vivo labeling with stable isotopes is necessary for the determination of turnover rates of metabolites. Dynamic metabolic profiling formed by the combination of metabolic profiling and stable isotope labeling technique enables to determine changes in metabolic flux exhaustively. In plants, responses to stress or environmental changes would be based on systematic variations in metabolism. The dynamic metabolic profiling would contribute to elucidate the complicated response mechanism.

紀要論文

Version of Record

1880-7593