Isolation and characterization of a novel chondroitin sulfate from squid liver integument rich in N-acetylgalactosamine(4,6-disulfate) and glucuronate(3-sulfate) residues

Shetty, Ajaya Kumar; Kobayashi, Takanari; Mizumoto, Shuji; Narumi, Masaki; Kudo, Yoshiaki; Yamada, Shuhei; Sugahara, Kazuyuki

Isolation and characterization of a novel chondroitin sulfate from squid liver integument rich in N-acetylgalactosamine(4,6-disulfate) and glucuronate(3-sulfate) residues

Shetty, Ajaya Kumar; Kobayashi, Takanari; Mizumoto, Shuji et al.

Carbohydrate Research, 2009, 344(12), 1526-1532

Permalink : https://hdl.handle.net/2115/39341

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アクセス権	open access
URI	https://hdl.handle.net/2115/39341
タイトル	Isolation and characterization of a novel chondroitin sulfate from squid liver integument rich in N-acetylgalactosamine(4,6-disulfate) and glucuronate(3-sulfate) residues
著者	Shetty, Ajaya Kumar ; Kobayashi, Takanari ; Mizumoto, Shuji ; Narumi, Masaki ; Kudo, Yoshiaki ; Yamada, Shuhei ; Sugahara, Kazuyuki
著者名 Shetty, Ajaya Kumar
著者名 Kobayashi, Takanari
著者名 Mizumoto, Shuji
著者名 Narumi, Masaki
著者名 Kudo, Yoshiaki
e-Rad_Researcher 70240017 著者名 Yamada, Shuhei
e-Rad_Researcher 60154449 著者名 Sugahara, Kazuyuki
言語	英語
キーワード	Chondroitin sulfate
	Glycosaminoglycan
	Growth factor
	Neurite outgrowth
	Squid liver
	Surface plasmon resonance
発行日	2009-08-17
出版者	Elsevier Ltd.
収録物名	Carbohydrate Research
巻(号)	344(12)
ページ	1526-1532
抄録	Novel chondroitin sulfate (CS) chains with an average molecular mass of 79.6 kDa were purified from squid liver integument. A compositional analysis of the CS chains using chondroitinases (CSases) ABC and AC-I revealed a range of variably sulfated disaccharides with GlcAβ1-3GalNAc(6-O-sulfate), GlcAβ1-3GalNAc(4-O-sulfate), and GlcAβ1-3GalNAc(4,6-O-disulfate) as the major ones, significant amounts of rare 3-O-sulfated GlcA-containing disaccharides, and a small amount of nonsulfated GlcAβ1-3GalNAc. The CS chains exhibited neurite outgrowth-promoting activity toward embryonic mouse hippocampal neurons, which was abolished completely by digestion with CSase ABC or AC-I. Consequently, whether these CS chains interact with heparin-binding growth factors was tested in a BIAcore system. All of the growth factors exhibited concentration-dependent and specific binding. CS chains from squid liver integument, with their unique composition and strong biological activities, may be a good candidate for therapeutic application.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0008-6215
関連情報	PMID 19344892
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.carres.2009.02.029