Structures of restriction endonuclease HindIII in complex with its cognate DNA and divalent cations

Watanabe, Nobuhisa; Takasaki, Yozo; Sato, Chika et al.

Acta Crystallographica. Section D, Biological Crystallography, 2009, 65(12), 1326-1333

Permalink : https://hdl.handle.net/2115/39986

このアイテムのアクセス数:1,435件（2026-08-19 21:57 集計）

ファイル	フォーマット	サイズ	閲覧回数	説明
watanabe_ActaCristD65	pdf	2.10 MB	8,125

アクセス権	open access
URI	https://hdl.handle.net/2115/39986
タイトル	Structures of restriction endonuclease HindIII in complex with its cognate DNA and divalent cations
著者	Watanabe, Nobuhisa ; Takasaki, Yozo ; Sato, Chika ; Ando, Shoji ; Tanaka, Isao
著者名 Watanabe, Nobuhisa
著者名 Takasaki, Yozo
著者名 Sato, Chika
著者名 Ando, Shoji
著者名 Tanaka, Isao
言語	英語
キーワード	restriction endonuclease
	DNA complex
	vaporizing iodine labeling method
発行日	2009-12
出版者	International Union of Crystallography
収録物名	Acta Crystallographica. Section D, Biological Crystallography
巻(号)	65(12)
ページ	1326-1333
抄録	The three-dimensional crystal structures of HindIII bound to its cognate DNA with and without divalent cations were solved at 2.17 and 2.00 Å resolution, respectively. HindIII forms a dimer. The structures showed that HindIII belongs to the EcoRI-like (α-class) subfamily of type II restriction endonucleases. The cognate DNA-complex structures revealed the specific DNA-recognition mechanism of HindIII by which it recognizes the palindromic sequence A/AGCTT. In the Mg2+ ion-soaked structure the DNAwas cleaved and two ions were bound at each active site, corresponding to the two-metal-ion mechanism.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	0907-4449
NCID	AA10885500
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1107/S0907444909041134