An experimental approach to study the biosynthesis of brominated metabolites by the red algal genus Laurencia

Suzuki, Minoru; Takahashi, Yoshinori; Nakano, Satoru; Abe, Tsuyoshi; Masuda, Michio; Ohnishi, Toshiyuki; Noya, Yoichi; Seki, Koh-ichi

An experimental approach to study the biosynthesis of brominated metabolites by the red algal genus Laurencia

Suzuki, Minoru; Takahashi, Yoshinori; Nakano, Satoru et al.

Phytochemistry, 2009, 70(11-12), 1410-1415

Permalink : https://hdl.handle.net/2115/42544

このアイテムのアクセス数:2,202件（2026-10-16 00:31 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
PHY70-11-12_1410-1415	pdf	216 KB	2,422

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/42544
タイトル	An experimental approach to study the biosynthesis of brominated metabolites by the red algal genus Laurencia
著者	Suzuki, Minoru ; Takahashi, Yoshinori ; Nakano, Satoru ; Abe, Tsuyoshi ; Masuda, Michio ; Ohnishi, Toshiyuki ; Noya, Yoichi ; Seki, Koh-ichi
著者名 Suzuki, Minoru
著者名 Takahashi, Yoshinori
著者名 Nakano, Satoru
著者名 Abe, Tsuyoshi
著者名 Masuda, Michio
著者名 Ohnishi, Toshiyuki
著者名 Noya, Yoichi
著者名 Seki, Koh-ichi
言語	英語
キーワード	Laurencia sp.
	Rhodomelaceae
	Brominated metabolites
	Biosynthesis
	82Br-compounds
	TLC-ARLG method
発行日	2009-07
出版者	Elsevier Ltd.
収録物名	Phytochemistry
巻(号)	70(11-12)
ページ	1410-1415
抄録	The production of labeled brominated metabolites with radioactive 82Br in Laurencia species was investigated as part of a study of the biosynthesis of halogenated metabolites from species belonging to the red algal genus Laurencia (Rhodomelaceae, Ceramiales). Radiobromide [82Br], thin-layer chromatography (TLC), and TLC-autoradioluminography (ARLG) were used. When cultured in artificial seawater medium (ASP12NTA including Na82Br) under 16:8 h light:dark (LD) illumination cycles for 24 h, each of the strains of Laurencia, Laurencia japonensis Abe et Masuda, Laurencia nipponica Yamada (laurencin-producing race and laureatin-producing race), and Laurencia okamurae Yamada, produced species- (or race-) specific 82Br-containing metabolites. In the case of the laurencin-producing race of L. nipponica, laurencin and deacetyllaurencin were found to be produced in approximately 1:1 ratio, though laurencin is the major metabolite in the wild sample. Furthermore, when cultured in the dark, the production rates of brominated metabolites in Laurencia spp. were found to be diminished. The present study strongly indicates that the use of radiobromine [82Br] in combination with the TLC-ARLG method is an effective approach for investigating the biosynthesis of brominated metabolites in Laurencia.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0031-9422
関連情報	PMID 19733868
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.phytochem.2009.07.038