Transformation of androgenic-derived Festulolium plants (Lolium perenne L. × Festuca pratensis Huds.) by Agrobacterium tumefaciens

Guo, Yang-Dong; Hisano, Hiroshi; Shimamoto, Yoshiya; Yamada, Toshihiko

Transformation of androgenic-derived Festulolium plants (Lolium perenne L. × Festuca pratensis Huds.) by Agrobacterium tumefaciens

Guo, Yang-Dong; Hisano, Hiroshi; Shimamoto, Yoshiya et al.

Plant Cell, Tissue and Organ Culture, 2009, 96(2), 219-227

Permalink : https://hdl.handle.net/2115/42593

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アクセス権	open access
URI	https://hdl.handle.net/2115/42593
タイトル	Transformation of androgenic-derived Festulolium plants (Lolium perenne L. × Festuca pratensis Huds.) by Agrobacterium tumefaciens
著者	Guo, Yang-Dong ; Hisano, Hiroshi ; Shimamoto, Yoshiya ; Yamada, Toshihiko
著者名 Guo, Yang-Dong
著者名 Hisano, Hiroshi
著者名 Shimamoto, Yoshiya
e-Rad_Researcher 70343952 著者名 Yamada, Toshihiko
言語	英語
キーワード	androgenic callus
	anther culture
	dihaploid
	GUS activity
	transgenic forage grass
発行日	2009-02
出版者	Springer
収録物名	Plant Cell, Tissue and Organ Culture
巻(号)	96(2)
ページ	219-227
抄録	Genetic transformation of androgenic-derived amphidiploid Festulolium plants (Lolium perenne L. x Festuca pratensis Huds., 2n=4x=28) by Agrobacterium tumefaciens has been achieved. Anther culture-induced calli of Festulolium "Bx351" were inoculated with Agrobacterium tumefaciens strain LBA4404 carrying pIG121-Hm encoding the hygromycin resistance (hph) and β-glucuronidase (uidA) genes under the control of a CaMV 35S promoter. Twenty-three putative transformants were obtained from the hygromycin selection, 19 of which (82.6%) showed GUS activity. The integration of transgenes was detected by using genomic DNA PCR analysis, RT-PCR analysis and Southern blot hybridization, respectively, which revealed that foreign gene was integrated into the genomes of dihaploid transformants (2n=2x=14). The haploid embryogenic system offers a stable means of transformation, as the introduced trait can be readily fixed through chromosome doubling.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	The original publication is available at www.springerlink.com
PISSN	0167-6857
EISSN	1573-5044
NCID	AA10472996
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s11240-008-9478-6