Evaluation of Echinococcus multilocularis tetraspanins as vaccine candidates against primary alveolar echinococcosis

Dang, Zhisheng; Yagi, Kinpei; Oku, Yuzaburo; Kouguchi, Hirokazu; Kajino, Kiichi; Watanabe, Junichi; Matsumoto, Jun; Nakao, Ryo; Wakaguri, Hiroyuki; Toyoda, Atsushi; Sugimoto, Chihiro

Evaluation of Echinococcus multilocularis tetraspanins as vaccine candidates against primary alveolar echinococcosis

Dang, Zhisheng; Yagi, Kinpei; Oku, Yuzaburo et al.

Vaccine, 2009, 27(52), 7339-7345

Permalink : https://hdl.handle.net/2115/42601

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アクセス権	open access
URI	https://hdl.handle.net/2115/42601
タイトル	Evaluation of Echinococcus multilocularis tetraspanins as vaccine candidates against primary alveolar echinococcosis
著者	Dang, Zhisheng ; Yagi, Kinpei ; Oku, Yuzaburo ; Kouguchi, Hirokazu ; Kajino, Kiichi ; Watanabe, Junichi ; Matsumoto, Jun ; Nakao, Ryo ; Wakaguri, Hiroyuki ; Toyoda, Atsushi ; Sugimoto, Chihiro
著者名 Dang, Zhisheng
著者名 Yagi, Kinpei
e-Rad_Researcher 60133716 著者名 Oku, Yuzaburo
e-Rad_Researcher 50435567 著者名 Kouguchi, Hirokazu
e-Rad_Researcher 80322147 著者名 Kajino, Kiichi
著者名 Watanabe, Junichi
著者名 Matsumoto, Jun
e-Rad_Researcher 50633955 著者名 Nakao, Ryo
著者名 Wakaguri, Hiroyuki
著者名 Toyoda, Atsushi
e-Rad_Researcher 90231373 著者名 Sugimoto, Chihiro
言語	英語
キーワード	Echinococcus multilocularis
	Tetraspanin
	Vaccine
	Oncosphere
	Primary alveolar echinococcosis
発行日	2009-12-09
出版者	Elsevier Ltd.
収録物名	Vaccine
巻(号)	27(52)
ページ	7339-7345
抄録	Echinococcus multilocularis causes an important zoonotic cestode disease. The metacestocle stage proliferates in the liver of intermediate hosts including human and rodents and forms multiple cysts. Recently, members of a transmembrane protein tetraspanin (TSP) family have been used as vaccines against schistosomosis, or as diagnostic antigens for cysticercosis. In this study, seven tetraspanins of E. multilocularis, designated as TSP1 to TSP7, were evaluated for their protective potential against primary alveolar echinococcosis. The large extracellular loop (LEL) region of these tetraspanins was cloned from a full-length enriched cDNA library of E. multilocularis metacestodes and expressed in Escherichia coli as a fusion protein with thioredoxin. Recombinant TSPs were applied as vaccines against an E. multilocularis primary experimental infection in BALB/c mice. Cyst lesions in the livers of vaccinated and non-vaccinated mice were counted. The cyst lesion reduction rates induced by the seven tetraspanins in vaccinated vis-à-vis non-vaccinated mice were: 87.9%, 65.8%, 85.1%, 66.9%, 73.7%, 72.9% and 37.6%. Vaccination conferred protective rates to mice ranging from 0% (TSP5, 6, 7) to maximally 33% (TSP1, 3). The results indicated that recombinant tetraspanins have varying protective effects against primary alveolar echinococcosis and could be used in vaccine development.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0264-410X
関連情報	PMID 19782112
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.vaccine.2009.09.045