open access

https://hdl.handle.net/2115/42677

Two distinct regions in Staphylococcus aureus GatCAB guarantee accurate tRNA recognition

英語

2010-01

Oxford University Press

Nucleic Acids Research

38(2)

672-682

In many prokaryotes the biosynthesis of the amide aminoacyl-tRNAs, Gln-tRNA^[Gln] and Asn-tRNA^[Asn], proceeds by an indirect route in which mischarged Glu-tRNA^[Gln] or Asp-tRNA^[Asn] is amidated to the correct aminoacyl-tRNA catalyzed by a tRNA-dependent amidotransferase (AdT). Two types of AdTs exist: bacteria, archaea and organelles possess heterotrimeric GatCAB, while heterodimeric GatDE occurs exclusively in archaea. Bacterial GatCAB and GatDE recognize the first base pair of the acceptor stem and the D-loop of their tRNA substrates, while archaeal GatCAB recognizes the tertiary core of the tRNA, but not the first base pair. Here, we present the crystal structure of the full-length Staphylococcus aureus GatCAB. Its GatB tail domain possesses a conserved Lys rich motif that is situated close to the variable loop in a GatCAB:tRNA^[Gln] docking model. This motif is also conserved in the tail domain of archaeal GatCAB, suggesting this basic region may recognize the tRNA variable loop to discriminate Asp-tRNA^[Asn] from Asp-tRNA^[Asp] in archaea. Furthermore, we identified a 3_[10] turn in GatB that permits the bacterial GatCAB to distinguish a U1-A72 base pair from a G1-C72 pair; the absence of this element in archaeal GatCAB enables the latter enzyme to recognize aminoacyl-tRNAs with G1-C72 base pairs.

学術雑誌論文

Version of Record

0305-1048