Influence of Medium Components and Growth Conditions on Pediocin Iz3.13 Production by Pediococcus pentosaceus Iz3.13, Isolated from Japanese Traditional Fermented Seafood

Kobayashi, Tetsuya; Yamazaki, Koji; Bagenda, Dominic K.; Kawai, Yuji

Influence of Medium Components and Growth Conditions on Pediocin Iz3.13 Production by Pediococcus pentosaceus Iz3.13, Isolated from Japanese Traditional Fermented Seafood

Kobayashi, Tetsuya; Yamazaki, Koji; Bagenda, Dominic K. et al.

北海道大学水産科学研究彙報, 2010, 60(1), 5-12

Permalink : https://hdl.handle.net/2115/42764

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アクセス権	open access
URI	https://hdl.handle.net/2115/42764
タイトル	Influence of Medium Components and Growth Conditions on Pediocin Iz3.13 Production by Pediococcus pentosaceus Iz3.13, Isolated from Japanese Traditional Fermented Seafood
著者	Kobayashi, Tetsuya ; Yamazaki, Koji ; Bagenda, Dominic K. ; Kawai, Yuji
著者名 Kobayashi, Tetsuya
e-Rad_Researcher 40250500 著者名 Yamazaki, Koji
著者名 Bagenda, Dominic K.
e-Rad_Researcher 60195039 著者名 Kawai, Yuji
言語	英語
キーワード	Bacteriocin
	Pediocin
	Pediococcus
	Medium components
	Culture condition
	Optimization
発行日	2010-03-09
出版者	北海道大学大学院水産科学研究院
出版者	Research Faculty of Fisheries Sciences, Hokkaido University
収録物名	北海道大学水産科学研究彙報
収録物名	Bulletin of Fisheries Sciences, Hokkaido University
巻(号)	60(1)
ページ	5-12
抄録	Growth requirements for maximum production of pediocin Iz3.13 by Pediococcus pentosaceus Iz3.13 were studied. Physical and chemical parameters that enhanced pediocin production included incubation in static broth at 30℃, with an initial pH of 4.5. Optimum nutritional conditions that enhanced pediocin Iz3.13 production were 3.5% Trypticase peptone, 3.5% Phytone peptone or 5.0% Proteose peptone No.3 as nitrogen sources, and glucose or maltose as carbon sources. Supplementing growth media with beef extract and MgCl2 also enhanced production. This study proposes an optimized growth medium containing 5.0% Proteose peptone No.3, 3.5% Phytone peptone, 2.5% glucose, 1.5% MgCl2, 3.0% beef extract and 0.25% K2HPO4. This medium significantly enhanced pediocin Iz3.13 production (5.1 fold increase) as compared to a basal medium.
資源タイプ	紀要論文
出版タイプ	Version of Record
PISSN	1346-1842