Contribution of cellular contractility to spatial and temporal variations in cellular stiffness

Nagayama, Masafumi; Haga, Hisashi; Takahashi, Masayuki; Saitoh, Takayuki; Kawabata, Kazushige

Contribution of cellular contractility to spatial and temporal variations in cellular stiffness

Nagayama, Masafumi; Haga, Hisashi; Takahashi, Masayuki et al.

Experimental Cell Research, 2004, 300(2), 396-405

Permalink : https://hdl.handle.net/2115/428

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アクセス権	open access
URI	https://hdl.handle.net/2115/428
タイトル	Contribution of cellular contractility to spatial and temporal variations in cellular stiffness
著者	Nagayama, Masafumi ; Haga, Hisashi ; Takahashi, Masayuki ; Saitoh, Takayuki ; Kawabata, Kazushige
e-Rad_Researcher 70374585 著者名 Nagayama, Masafumi 永山, 昌史
e-Rad_Researcher 00292045 著者名 Haga, Hisashi 芳賀, 永
e-Rad_Researcher 50241295 著者名 Takahashi, Masayuki 高橋, 正行
著者名 Saitoh, Takayuki 斉藤, 隆幸
著者名 Kawabata, Kazushige 川端, 和重
言語	英語
キーワード	Fibroblast
	Scanning probe microscopy
	Atomic force microscopy
	Lysophosphatidic acid
	Y-27632
	Stress fiber
	Actomyosin
	Mechanical stability
発行日	2004-11-01
出版者	Elsevier Inc.
収録物名	Experimental Cell Research
巻(号)	300(2)
ページ	396-405
抄録	Scanning probe microscopy and immunofluorescence observations indicated that cellular stiffness was attributed to a contractile network structure consisting of stress fibers. We measured temporal variations in cellular stiffness when cellular contractility was regulated by dosing with lysophosphatidic acid or Y-27632. This experiment reveals a clear relation between cellular stiffness and contractility: Increases in contractility cause cells to stiffen. On the other hand, decreases in contractility reduce cellular stiffness. In both cases, not only the stiffness of the stress fibers but also that of the whole of the cell varies. Immunofluorescence observations of myosin II and vinculin indicated that the stiffness variations induced by the regulation of cellular contractility were mainly due to rearrangements of the contractile actin network on the dorsal surface. Taken together, our findings provide evidence that the actin cytoskeletal network and its contractility features provide and modulate the mechanical stability of adherent cells.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	00144827
関連情報	PMID 15475004
関連情報	URI http://www.sciencedirect.com/science/journal/00144827
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.yexcr.2004.07.034