open access

https://hdl.handle.net/2115/43011

Difructose anhydride III promotes iron absorption in the rat large intestine

英語

Divalent cation transporter-1

2010-01

Elsevier

Nutrition

26(1)

120-127

Objectives: We examined the intestinal segment responsible for and the mechanisms involved in the nondigestible disaccharide difructose anhydride III (DFA) III-mediated enhancement of iron absorption. Methods: Net iron and calcium absorption (%) were observed in male SD rats fed an AIN93G-based diet with or without DFAIII (30 g/kg diet) for 2 weeks after cecectomy or laparotomy (Exp. 1). The absorption rates of calcium and iron from ligated jejunal loops (Exp. 2) or ligated cecal sacs (Exp. 3) in anesthetized rats fed a control or DFA diet were observed with or without the addition of DFAIII to the instilled mucosal fluid. Levels of the major iron transporter DMT-1 mRNA per 28S rRNA were evaluated by real-time PCR in the intestinal mucosa. Results: Net absorption (%) of iron and calcium was higher in the DFA group than in the DFA-free group in laparotomized rats. The enhancement of iron absorption was almost and calcium absorption was partly abolished by cecectomy. The absorption rate of calcium, but not iron, from the jejunal loops was increased by the addition of DFAIII (100 mmol/L). Iron absorption was clearly higher in the ligated cecal sacs of the DFAIII-fed group than in the DFA-free group with or without the mucosal addition of DFAIII. DMT-1 mRNA level per 28S rRNA was maintained in the cecal mucosa through large increases in cecal size due to DFAIII feeding. Conclusion: DFAIII-induced increases in iron absorption are the result of increased cecal iron absorptive capacity through expansion of the cecal mucosa maintaining DMT-1 mRNA expression.

学術雑誌論文

Accepted Manuscript

0899-9007