pfaB products determine the molecular species produced in bacterial polyunsaturated fatty acid biosynthesis

Orikasa, Yoshitake; Tanaka, Mika; Sugihara, Shinji et al.

FEMS Microbiology Letters, 2009, 295(2), 170-176

Permalink : https://hdl.handle.net/2115/43086

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アクセス権
open access
URI
https://hdl.handle.net/2115/43086
タイトル
pfaB products determine the molecular species produced in bacterial polyunsaturated fatty acid biosynthesis
著者
Orikasa, Yoshitake ; Tanaka, Mika ; Sugihara, Shinji ; Hori, Ryuji ; Nishida, Takanori ; Ueno, Akio ; Morita, Naoki ; Yano, Yutaka ; Yamamoto, Kouhei ; Shibahara, Akira ; Hayashi, Hidenori ; Yamada, Yohko ; Yamada, Akiko ; Yu, Reiko ; Watanabe, Kazuo ; Okuyama, Hidetoshi
著者名
Orikasa, Yoshitake
著者名
Tanaka, Mika
著者名
Sugihara, Shinji
著者名
Hori, Ryuji
著者名
Nishida, Takanori
著者名
Ueno, Akio
著者名
Morita, Naoki
著者名
Yano, Yutaka
著者名
Yamamoto, Kouhei
著者名
Shibahara, Akira
著者名
Hayashi, Hidenori
著者名
Yamada, Yohko
著者名
Yamada, Akiko
著者名
Yu, Reiko
著者名
Watanabe, Kazuo
e-Rad_Researcher
90125295
著者名
Okuyama, Hidetoshi
言語
英語
キーワード
docosahexaenoic acid
eicosapentaenoic acid
Moritella marina strain MP-1
polyunsaturated fatty acid
pfaB gene
発行日
2009-06
出版者
Blackwell Publishing
収録物名
FEMS Microbiology Letters
巻(号)
295(2)
ページ
170-176
抄録
When pDHA4, a vector carrying all five pfaA-pfaE genes responsible for docosahexaenoic acid (DHA; 22:6) biosynthesis in Moritella marina MP-1, was coexpressed in Escherichia coli with the individual pfaA-pfaD genes for eicosapentaenoic acid (EPA; 20:5) biosynthesis from Shewanella pneumatophori SCRC-2738, both polyunsaturated fatty acids were synthesized only in the recombinant carrying pfaB for EPA synthesis. Escherichia coli coexpressing a deleted construct comprising pfaA, pfaC, pfaD and pfaE for EPA and pfaB for DHA produced EPA and DHA. Both EPA and DHA were detected in bacteria that inherently contained pfa genes for DHA. These results suggest that PfaB is the key enzyme determining the final product in EPA or DHA biosynthesis.
資源タイプ
学術雑誌論文
出版タイプ
Accepted Manuscript
権利情報
The definitive version is available at www.blackwell-synergy.com
PISSN
0378-1097
関連情報
PMID
19453514
関連情報 (isVersionOf)
出版社版DOI
https://doi.org/10.1111/j.1574-6968.2009.01582.x