Fluorescence lifetime images of green fluorescent protein in HeLa cells during TNF-α induced apoptosis

Ito, Toshiyuki; Oshita, Shugo; Nakabayashi, Takakazu; Sun, Fan; Kinjo, Masataka; Ohta, Nobuhiro

Fluorescence lifetime images of green fluorescent protein in HeLa cells during TNF-α induced apoptosis

Ito, Toshiyuki; Oshita, Shugo; Nakabayashi, Takakazu et al.

Photochemical & Photobiological Sciences, 2009, 8(6), 763-767

Permalink : https://hdl.handle.net/2115/43088

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アクセス権	open access
URI	https://hdl.handle.net/2115/43088
タイトル	Fluorescence lifetime images of green fluorescent protein in HeLa cells during TNF-α induced apoptosis
著者	Ito, Toshiyuki ; Oshita, Shugo ; Nakabayashi, Takakazu ; Sun, Fan ; Kinjo, Masataka ; Ohta, Nobuhiro
著者名 Ito, Toshiyuki
著者名 Oshita, Shugo
著者名 Nakabayashi, Takakazu
著者名 Sun, Fan
e-Rad_Researcher 70177971 著者名 Kinjo, Masataka
著者名 Ohta, Nobuhiro
言語	英語
キーワード	Fluorescence lifetime imaging (FLIM)
	Apoptosis
	HeLa cell
	Enhanced green fluorescent protein (EGFP)
	Tumor necrosis factor-α (TNF-α)
	Cycloheximide
発行日	2009-06
出版者	Royal Society of Chemistry
収録物名	Photochemical & Photobiological Sciences
巻(号)	8(6)
ページ	763-767
抄録	Fluorescence lifetime images of HeLa cells expressing enhanced green fluorescent protein (EGFP) have been measured, as apoptosis is induced by tumor necrosis factor-α (TNF-α) in combination with cycloheximide. The fluorescence lifetime of EGFP is found to decrease after the induction of apoptosis, indicating that the change in environment occurs around the chromophore of EGFP with the apoptosis process. The fluorescence lifetime imaging technique can be used to perform in vivo observation of cell death processes. Fluorescence lifetime measurements are useful to examine the induction of the apoptosis process, even when a morphological change of each cell cannot be observed because of a low spatial resolution.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Photochem. Photobiol. Sci., 2009, 8, 763-767 - Reproduced by permission of The Royal Society of Chemistry (RSC)
PISSN	1474-905X
関連情報	PMID 19492103
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1039/b902341k