A novel copper(II) coordination at His186 in full-length murine prion protein

Watanabe, Yasuko; Hiraoka, Wakako; Igarashi, Manabu; Ito, Kimihito; Shimoyama, Yuhei; Horiuchi, Motohiro; Yamamori, Tohru; Yasui, Hironobu; Kuwabara, Mikinori; Inagaki, Fuyuhiko; Inanami, Osamu

A novel copper(II) coordination at His186 in full-length murine prion protein

Watanabe, Yasuko; Hiraoka, Wakako; Igarashi, Manabu et al.

Biochemical and Biophysical Research Communications, 2010, 394(3), 522-528

Permalink : https://hdl.handle.net/2115/43092

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アクセス権	open access
URI	https://hdl.handle.net/2115/43092
タイトル	A novel copper(II) coordination at His186 in full-length murine prion protein
著者	Watanabe, Yasuko ; Hiraoka, Wakako ; Igarashi, Manabu ; Ito, Kimihito ; Shimoyama, Yuhei ; Horiuchi, Motohiro ; Yamamori, Tohru ; Yasui, Hironobu ; Kuwabara, Mikinori ; Inagaki, Fuyuhiko ; Inanami, Osamu
著者名 Watanabe, Yasuko
著者名 Hiraoka, Wakako
e-Rad_Researcher 10374240 著者名 Igarashi, Manabu
e-Rad_Researcher 60396314 著者名 Ito, Kimihito
著者名 Shimoyama, Yuhei
e-Rad_Researcher 30219216 著者名 Horiuchi, Motohiro
e-Rad_Researcher 00512675 著者名 Yamamori, Tohru
e-Rad_Researcher 10570228 著者名 Yasui, Hironobu
著者名 Kuwabara, Mikinori
e-Rad_Researcher 70011757 著者名 Inagaki, Fuyuhiko
e-Rad_Researcher 10193559 著者名 Inanami, Osamu
言語	英語
キーワード	Prion protein
	Cu(II)
	C-terminal domain
	ESR
	SDSL
	MTSSL
	Interspin distance
	Dipolar interaction
	Histidine residue
発行日	2010-04-09
出版者	Elsevier
収録物名	Biochemical and Biophysical Research Communications
巻(号)	394(3)
ページ	522-528
抄録	To explore Cu(II) ion coordination by His186 in the C-terminal domain of full-length prion protein (moPrP), we utilized the magnetic dipolar interaction between a paramagnetic metal, Cu(II) ion, and a spin probe introduced in the neighborhood of the postulated binding site by the spin labeling technique (SDSL technique). Six moPrP mutants, moPrP(D143C), moPrP(Y148C), moPrP(E151C), moPrP(Y156C), moPrP(T189C), and moPrP(Y156C,H186A), were reacted with a methane thiosulfonate spin probe and a nitroxide residue (R1) was created in the binding site of each one. Line broadening of the ESR spectra was induced in the presence of Cu(II) ions in moPrP(Y148R1), moPrP(Y151R1), moPrP(Y156R1), and moPrP( T189R1) but not moPrP(D143R1). This line broadening indicated the presence of electron–electron dipolar interaction between Cu(II) and the nitroxide spin probe, suggesting that each interspin distance was within 20 Å. The interspin distance ranges between Cu(II) and the spin probes of moPrP(Y148R1), moPrP(Y151R1), moPrP(Y156R1), and moPrP(T189R1) were estimated to be 12.1 Å, 18.1 Å, 10.7 Å, and 8.4 Å, respectively. In moPrP(Y156R1,H186A), line broadening between Cu(II) and the spin probe was not observed. These results suggest that a novel Cu(II) binding site is involved in His186 in the Helix2 region of the C-terminal domain of moPrPC.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0006-291X
EISSN	1090-2104
NCID	AA00564395
関連情報	PMID 20206606
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.bbrc.2010.03.003