DOCK2 regulates cell proliferation through Rac and ERK activation in B cell lymphoma

Wang, Lei; Nishihara, Hiroshi; Kimura, Taichi; Kato, Yasutaka; Tanino, Mishie; Nishio, Mitsufumi; Obara, Masato; Endo, Tomoyuki; Koike, Takao; Tanaka, Shinya

DOCK2 regulates cell proliferation through Rac and ERK activation in B cell lymphoma

Wang, Lei; Nishihara, Hiroshi; Kimura, Taichi et al.

Biochemical and Biophysical Research Communications, 2010, 395(1), 111-115

Permalink : https://hdl.handle.net/2115/43094

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アクセス権	open access
URI	https://hdl.handle.net/2115/43094
タイトル	DOCK2 regulates cell proliferation through Rac and ERK activation in B cell lymphoma
著者	Wang, Lei ; Nishihara, Hiroshi ; Kimura, Taichi ; Kato, Yasutaka ; Tanino, Mishie ; Nishio, Mitsufumi ; Obara, Masato ; Endo, Tomoyuki ; Koike, Takao ; Tanaka, Shinya
著者名 Wang, Lei
e-Rad_Researcher 50322805 著者名 Nishihara, Hiroshi
著者名 Kimura, Taichi
著者名 Kato, Yasutaka
著者名 Tanino, Mishie
著者名 Nishio, Mitsufumi
著者名 Obara, Masato
著者名 Endo, Tomoyuki
著者名 Koike, Takao
著者名 Tanaka, Shinya
言語	英語
キーワード	DOCK2
	B cell lymphoma
	Rac
	ERK
	Hematopoietic matignancy
発行日	2010-04-23
出版者	Elsevier
収録物名	Biochemical and Biophysical Research Communications
巻(号)	395(1)
ページ	111-115
抄録	DOCK2; a member of the CDM protein family, regulates cell motility and cytokine production through the activation of Rac in mammalian hematopoietic cells and plays a pivotal role in the modulation of the immune system. Here we demonstrated the alternative function of DOCK2 in hematopoietic tumor cells, especially in terms of its association with the tumor progression. Immunostaining for DOCK2 in 20 cases of human B cell lymphoma tissue specimens including diffuse large B cell lymphoma and follicular lymphoma revealed the prominent expression of DOCK2 in all of the lymphoma cells. DOCK2-knockdown (KD) of the B cell lymphoma cell lines, Ramos and Raji, using the lentiviral shRNA system presented decreased cell proliferation compared to the control cells. Furthermore, the tumor formation of DOCK2-KD Ramos cell in nude mice was significantly abrogated. Western blotting analysis and pull-down assay using GST-PAK-RBD kimeric protein suggested the presence of DOCK2-Rac-ERK pathway regulating the cell proliferation of these lymphoma cells. This is the first report to clarify the prominent role of DOCK2 in hematopoietic malignancy.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0006-291X
関連情報	PMID 20350533
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.bbrc.2010.03.148