Flow cytometric analysis of the contributing factors for antimicrobial activity enhancement of cell-penetrating type peptides : Case study on engineered apidaecins

Matsumoto, Ken'ichiro; Orikasa, Yoshitake; Ichinohe, Kenta; Hashimoto, Shigeki; Ooi, Toshihiko; Taguchi, Seiichi

Flow cytometric analysis of the contributing factors for antimicrobial activity enhancement of cell-penetrating type peptides : Case study on engineered apidaecins

Matsumoto, Ken'ichiro; Orikasa, Yoshitake; Ichinohe, Kenta et al.

Biochemical and Biophysical Research Communications, 2010, 395(1), 7-10

Permalink : https://hdl.handle.net/2115/43104

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アクセス権	open access
URI	https://hdl.handle.net/2115/43104
タイトル	Flow cytometric analysis of the contributing factors for antimicrobial activity enhancement of cell-penetrating type peptides : Case study on engineered apidaecins
著者	Matsumoto, Ken'ichiro ; Orikasa, Yoshitake ; Ichinohe, Kenta ; Hashimoto, Shigeki ; Ooi, Toshihiko ; Taguchi, Seiichi
e-Rad_Researcher 80360642 著者名 Matsumoto, Ken'ichiro
著者名 Orikasa, Yoshitake
著者名 Ichinohe, Kenta
著者名 Hashimoto, Shigeki
e-Rad_Researcher 40223713 著者名 Ooi, Toshihiko
e-Rad_Researcher 70216828 著者名 Taguchi, Seiichi
言語	英語
キーワード	Cell penetration
	Flow cytometry
	Antibacterial peptide
	Honey bee
発行日	2010-04-23
出版者	Elsevier
収録物名	Biochemical and Biophysical Research Communications
巻(号)	395(1)
ページ	7-10
抄録	Contributing factors for the antimicrobial activity enhancement of N-terminally engineered mutants of cell-penetrating apidaecins were analyzed based on their cell-penetration efficiency. The flow cytometric analysis of the engineered apidaecins labeled with carboxyfluorescein (FAM) revealed their enhanced cell-penetrating efficiencies into Escherichia coli that should be one of key factors causing the enhanced antimicrobial activity. It is noteworthy that, for one mutant, the enhancement in antimicrobial activity (18-fold higher than wild type) was greater than that of cell penetration (5.9-fold), suggesting that the N-terminal mutation may reinforce both interaction with unidentified intracellular target(s) and cell-penetration efficiency.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0006-291X
関連情報	PMID 20302844
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.bbrc.2010.03.088