DNA Microarray Analysis of Whole Blood Cells and Insulin-Sensitive Tissues Reveals the Usefulness of Blood RNA Profiling as a Source of Markers for Predicting Type 2 Diabetes

Hayashi, Yasuhiro; Kajimoto, Kazuaki; Iida, Shinya; Sato, Yuichiro; Mizufune, Shogo; Kaji, Noritada; Kamiya, Hiroyuki; Baba, Yoshinobu; Harashima, Hideyoshi

DNA Microarray Analysis of Whole Blood Cells and Insulin-Sensitive Tissues Reveals the Usefulness of Blood RNA Profiling as a Source of Markers for Predicting Type 2 Diabetes

Hayashi, Yasuhiro; Kajimoto, Kazuaki; Iida, Shinya et al.

Biological & Pharmaceutical Bulletin, 2010, 33(6), 1033-1042

Permalink : https://hdl.handle.net/2115/43118

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アクセス権	open access
URI	https://hdl.handle.net/2115/43118
タイトル	DNA Microarray Analysis of Whole Blood Cells and Insulin-Sensitive Tissues Reveals the Usefulness of Blood RNA Profiling as a Source of Markers for Predicting Type 2 Diabetes
著者	Hayashi, Yasuhiro ; Kajimoto, Kazuaki ; Iida, Shinya ; Sato, Yuichiro ; Mizufune, Shogo ; Kaji, Noritada ; Kamiya, Hiroyuki ; Baba, Yoshinobu ; Harashima, Hideyoshi
著者名 Hayashi, Yasuhiro
著者名 Kajimoto, Kazuaki
著者名 Iida, Shinya
著者名 Sato, Yuichiro
著者名 Mizufune, Shogo
著者名 Kaji, Noritada
著者名 Kamiya, Hiroyuki
著者名 Baba, Yoshinobu
著者名 Harashima, Hideyoshi
言語	英語
キーワード	whole blood cell
	type 2 diabetes mellitus
	gene expression
	DNA microarray
	Otsuka Long-Evans Tokushima Fatty rat
	diagnosis
発行日	2010-06
出版者	Pharmaceutical Society of Japan
収録物名	Biological & Pharmaceutical Bulletin
巻(号)	33(6)
ページ	1033-1042
抄録	To determine if gene expression profiling of whole blood cells is a useful source of markers for the early diagnosis of the onset of type 2 diabetes, we examined gene expression profiling of whole blood cells and type 2 diabetes-related organs, such as liver, adipose tissue, and skeletal muscle, of Otsuka Long-Evans Tokushima Fatty (OLETF) rats. At the age of 6 weeks, RNA was isolated from tissues of fasted OLETF and control Long-Evans Tokushima Otsuka (LETO) rats. Gene expression was analyzed using the Agilent rat oligo microarray. Gene ontology analysis showed that gene expression of biologically meaningful groups of genes in liver, adipose tissue, and skeletal muscle, which are involved in the pathogenesis of type 2 diabetes, differed between OLETF and LETO rats. Three hundred genes of whole blood cells were differentially expressed. Four out of these 300 genes were related to the insulin-signaling pathway and 57 out of 300 genes were up- or down-regulated in at least one tissues in OLETF rats. These results support our hypothesis that gene expression profiling of whole blood cells might be a useful source of markers to predict the onset of type 2 diabetes.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	0918-6158
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1248/bpb.33.1033