Transgene expression efficiency from plasmid DNA delivered as a complex with histone H3

Kamiya, Hiroyuki; Goto, Hitomi; Kanda, Genki et al.

International Journal of Pharmaceutics, 2010, 392(1-2), 249-253

Permalink : https://hdl.handle.net/2115/43144

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アクセス権	open access
URI	https://hdl.handle.net/2115/43144
タイトル	Transgene expression efficiency from plasmid DNA delivered as a complex with histone H3
著者	Kamiya, Hiroyuki ; Goto, Hitomi ; Kanda, Genki ; Yamada, Yuma ; Harashima, Hideyoshi
著者名 Kamiya, Hiroyuki
著者名 Goto, Hitomi
著者名 Kanda, Genki
著者名 Yamada, Yuma
e-Rad_Researcher 00183567 著者名 Harashima, Hideyoshi
言語	英語
キーワード	Exogenous DNA
	Histone
	H3
	Intranuclear disposition
	Expression efficiency
発行日	2010-06-15
出版者	Elsevier B.V.
収録物名	International Journal of Pharmaceutics
巻(号)	392(1-2)
ページ	249-253
抄録	The intranuclear disposition of plasmid DNA is extremely important for transgene expression. Exogenous histones have been used as carriers of plasmid DNA in histone-mediated gene delivery. In this study, the effects of exogenous histone H3 complexed with plasmid DNA on transgene expression efficiency were examined. The plasmid-histone complexes in various ratios were transfected into HeLa cells by osmotic pressure. Histone H3 suppressed transgene expression in the nucleus in a dose-dependent manner. Our results suggest that the histone-mediated gene delivery is unlikely to be useful, from the viewpoint of the intranuclear disposition.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0378-5173
関連情報	PMID 20362041
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.ijpharm.2010.03.035