Integrin β1-dependent invasive migration of irradiation-tolerant human lung adenocarcinoma cells in 3D collagen matrix

Ishihara, Seiichiro; Haga, Hisashi; Yasuda, Motoaki; Mizutani, Takeomi; Kawabata, Kazushige; Shirato, Hiroki; Nishioka, Takeshi

Integrin β1-dependent invasive migration of irradiation-tolerant human lung adenocarcinoma cells in 3D collagen matrix

Ishihara, Seiichiro; Haga, Hisashi; Yasuda, Motoaki et al.

Biochemical and Biophysical Research Communications, 2010, 396(3), 651-655

Permalink : https://hdl.handle.net/2115/43148

このアイテムのアクセス数:1,313件（2026-05-20 19:32 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
BBRC396-3_651-655	pdf	877 KB	1,338

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/43148
タイトル	Integrin β1-dependent invasive migration of irradiation-tolerant human lung adenocarcinoma cells in 3D collagen matrix
著者	Ishihara, Seiichiro ; Haga, Hisashi ; Yasuda, Motoaki ; Mizutani, Takeomi ; Kawabata, Kazushige ; Shirato, Hiroki ; Nishioka, Takeshi
著者名 Ishihara, Seiichiro
著者名 Haga, Hisashi
e-Rad_Researcher 90239765 著者名 Yasuda, Motoaki
著者名 Mizutani, Takeomi
著者名 Kawabata, Kazushige
著者名 Shirato, Hiroki
著者名 Nishioka, Takeshi
言語	英語
キーワード	Lung adenocarcinoma
	Irradiation
	3D culture
	Collagen gel
	Invasion
	Integrin β1
発行日	2010-06-04
出版者	Elsevier
収録物名	Biochemical and Biophysical Research Communications
巻(号)	396(3)
ページ	651-655
抄録	Radiotherapy is one of the effective therapies used for treating various malignant tumors. However, the emergence of tolerant cells after irradiation remains problematic due to their high metastatic ability, sometimes indicative of poor prognosis. In this study, we showed that subcloned human lung adenocarcinoma cells (A549P-3) that are irradiation-tolerant indicate high invasive activity in vitro, and exhibit an integrin β1 activity-dependent migratory pattern. In collagen gel overlay assay, majority of the A549P-3 cells displayed round morphology and low migration activity, whereas a considerable number of A549P-3IR cells surviving irradiation displayed a spindle morphology and high migration rate. Blocking integrin β1 activity reduced the migration rate of A549P-3IR cells and altered the cell morphology allowing them to assume a round shape. These results suggest that the A549P-3 cells surviving irradiation acquire a highly invasive integrin β1-dependent phenotype, and integrin β1 might be a potentially effective therapeutic target in combination with radiotherapy.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0006-291X
関連情報	PMID 20438698
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.bbrc.2010.04.150