Involvement of chondroitin sulfate E in the liver tumor focal formation of murine osteosarcoma cells

Basappa; Murugan, Sengottuvelan; Sugahara, Kazuki N.; Lee, Chun Man; ten Dam, Gerdy B.; van Kuppevelt, Toin H.; Miyasaka, Masayuki; Yamada, Shuhei; Sugahara, Kazuyuki

Involvement of chondroitin sulfate E in the liver tumor focal formation of murine osteosarcoma cells

Basappa; Murugan, Sengottuvelan; Sugahara, Kazuki N. et al.

Glycobiology, 2009, 19(7), 735-742

Permalink : https://hdl.handle.net/2115/43174

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アクセス権	open access
URI	https://hdl.handle.net/2115/43174
タイトル	Involvement of chondroitin sulfate E in the liver tumor focal formation of murine osteosarcoma cells
著者	Basappa ; Murugan, Sengottuvelan ; Sugahara, Kazuki N. ; Lee, Chun Man ; ten Dam, Gerdy B. ; van Kuppevelt, Toin H. ; Miyasaka, Masayuki ; Yamada, Shuhei ; Sugahara, Kazuyuki
著者名 Basappa
著者名 Murugan, Sengottuvelan
e-Rad_Researcher 60154449 著者名 Sugahara, Kazuki N.
著者名 Lee, Chun Man
著者名 ten Dam, Gerdy B.
著者名 van Kuppevelt, Toin H.
著者名 Miyasaka, Masayuki
著者名 Yamada, Shuhei
著者名 Sugahara, Kazuyuki
言語	英語
キーワード	chondroitin sulfate
	glycosaminoglycan
	tumor
	osteosarcoma
	sulfation
発行日	2009-07
出版者	Oxford University Press
収録物名	Glycobiology
巻(号)	19(7)
ページ	735-742
抄録	Cell surface heparan sulfate plays a critical role in regulating the metastatic behavior of tumor cells, whereas the role of chondroitin sulfate/dermatan sulfate (CS/DS) has been little understood in this context. Here, we characterized CS/DS chains from the murine osteosarcoma cell line LM8G7, which forms tumor nodules in liver. Structural analysis of the CS/DS chains showed a higher proportion of GlcUAβ1-3GalNAc(4,6-O-disulfate) (E-units) in LM8G7 (12%) than in its parental cell line LM8 (6%), which rarely forms tumors in the liver. Immunostaining with GD3G7, an antibody specific to E-units, confirmed the higher expression of the epitope in LM8G7 than LM8 cells. The tumor focal formation of LM8G7 cells in the liver in mice was effectively inhibited by the pre-administration of CS-E (rich in E-unit) or the pre-incubation of the antibody GD3G7 with the tumor cells. CS-E or GD3G7 inhibited the adhesion of LM8G7 cells to a laminin-coated plate in vitro. In addition, the invasive ability of LM8G7 cells in vitro was also reduced by the addition of CS-E or the antibody. Further, CS-E or the antibody inhibited the proliferation of LM8G7 cells dose-dependently. The binding of LM8G7 cells to VEGF in vitro was also significantly reduced by CS-E and GD3G7. Thus, the present study reveals the significance of highly sulfated CS/DS structures in the liver colonization of osteosarcoma cells and also provides a framework for the development of GAG-based anti-cancer molecules.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Glycobiology following peer review. The definitive publisher-authenticated version 19(7):735-742, July 2009 is available online at: http://dx.doi.org/10.1093/glycob/cwp041
PISSN	0959-6658
関連情報	PMID 19293233
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1093/glycob/cwp041