Cytotoxic Effect of Drosophila Deoxynucleoside Kinase Gene on Replicating Plasmid in HeLa Cells

Ito, Mana; Suda, Yusuke; Harashima, Hideyoshi et al.

Biological & Pharmaceutical Bulletin, 2010, 33(7), 1223-1227

Permalink : https://hdl.handle.net/2115/43201

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アクセス権
open access
URI
https://hdl.handle.net/2115/43201
タイトル
Cytotoxic Effect of Drosophila Deoxynucleoside Kinase Gene on Replicating Plasmid in HeLa Cells
著者
Ito, Mana ; Suda, Yusuke ; Harashima, Hideyoshi ; Kamiya, Hiroyuki
著者名
Ito, Mana
著者名
Suda, Yusuke
著者名
Harashima, Hideyoshi
著者名
Kamiya, Hiroyuki
言語
英語
キーワード
replicating plasmid
deoxynucleoside kinase
cell death
発行日
2010-07-01
収録物名
Biological & Pharmaceutical Bulletin
巻(号)
33(7)
ページ
1223-1227
抄録
To enhance the levels of transgene expression from plasmid-based nonviral vectors, replicating plasmids containing the SV40 origin and the SV40 large T antigen gene, as a model replicating unit, were constructed. The replicating luciferase plasmid DNA produced the luciferase protein more efficiently than the non-replicating luciferase plasmid DNA, as expected. Surprisingly, the introduction of the replicating plasmid DNA containing the Drosophila melanogaster deoxyribonucleoside kinase (Dm-dNK) gene was highly cytotoxic and caused cell death without nucleoside analogs. Our results confirm that transgenes on a replicating plasmid represent an excellent tool for effective protein production and suggest that efficient production of the Dm-dNK protein in tumor cells could be an attractive cancer therapy.
資源タイプ
学術雑誌論文
出版タイプ
Version of Record
PISSN
0918-6158
EISSN
1347-5215
関連情報 (isIdenticalTo)
出版社版DOI
https://doi.org/10.1248/bpb.33.1223