Isolation and culture of microvascular endothelial cells from murine inguinal and epididymal adipose tissues

Kajimoto, Kazuaki; Hossen, Md. Nazir; Hida, Kyoko; Ohga, Noritaka; Akita, Hidetaka; Hyodo, Mamoru; Hida, Yasuhiro; Harashima, Hideyoshi

Isolation and culture of microvascular endothelial cells from murine inguinal and epididymal adipose tissues

Kajimoto, Kazuaki; Hossen, Md. Nazir; Hida, Kyoko et al.

Journal of Immunological Methods, 2010, 357(1-2), 43-50

Permalink : https://hdl.handle.net/2115/43265

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/43265
タイトル	Isolation and culture of microvascular endothelial cells from murine inguinal and epididymal adipose tissues
著者	Kajimoto, Kazuaki ; Hossen, Md. Nazir ; Hida, Kyoko ; Ohga, Noritaka ; Akita, Hidetaka ; Hyodo, Mamoru ; Hida, Yasuhiro ; Harashima, Hideyoshi
著者名 Kajimoto, Kazuaki
著者名 Hossen, Md. Nazir
著者名 Hida, Kyoko
著者名 Ohga, Noritaka
著者名 Akita, Hidetaka
著者名 Hyodo, Mamoru
著者名 Hida, Yasuhiro
著者名 Harashima, Hideyoshi
言語	英語
キーワード	Endothelial cell
	Adipose tissue
	Isolation
	Primary culture
発行日	2010-05-31
出版者	Elsevier B.V.
収録物名	Journal of Immunological Methods
巻(号)	357(1-2)
ページ	43-50
抄録	Adipose tissue has long been considered to be a simple tissue that contains adipocytes. Because of this, the isolation and characterization of microvascular endothelical cells, which are also present in adipose tissue, have been neglected, even though they are components of a capillary network that surrounds each individual adipocyte. Here we report on a protocol for producing highly purified murine microvascular endothelial cells (MECs) from diverse sites of murine adipose tissues including inguinal and epididymal adipose tissues. The method is based on a combination of negative and positive immunomagnetic selection. The protocol involves the preparation of a single cell suspension (digestion, filtration and density gradient centrifugation), immunomagnetic enrichment of the CD45- cell population and the purification of the MECs by a combination of common specific markers CD31, CD102 and isolectin B4. The isolated MECs can be successively cultured for 10 to 12 passages without any detectable changes in morphology and phenotype. Therefore, the method described herein represents a protocol for the isolation and long-term maintenance of highly pure mouse MECs in high yields from adipose tissues.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0022-1759
関連情報	PMID 20307543
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.jim.2010.03.011