Molecular cloning and characterization of the AVR-Pia locus from a Japanese field isolate of Magnaporthe oryzae.

Miki, Shinsuke; Matsui, Kotaro; Kito, Hideki et al.

Molecular Plant Pathology, 2009, 10(3), 361-374

Permalink : https://hdl.handle.net/2115/43305

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アクセス権
open access
URI
https://hdl.handle.net/2115/43305
タイトル
Molecular cloning and characterization of the AVR-Pia locus from a Japanese field isolate of Magnaporthe oryzae.
著者
Miki, Shinsuke ; Matsui, Kotaro ; Kito, Hideki ; Otsuka, Keisuke ; Ashizawa, Taketo ; Yasuda, Nobuko ; Fukiya, Satoru ; Sato, Junko ; Hirayae, Kazuyuki ; Fujita, Yoshikatsu ; Nakajima, Toshihiko ; Tomita, Fusao ; Sone, Teruo
著者名
Miki, Shinsuke
著者名
Matsui, Kotaro
著者名
Kito, Hideki
著者名
Otsuka, Keisuke
著者名
Ashizawa, Taketo
著者名
Yasuda, Nobuko
著者名
Fukiya, Satoru
著者名
Sato, Junko
著者名
Hirayae, Kazuyuki
著者名
Fujita, Yoshikatsu
著者名
Nakajima, Toshihiko
著者名
Tomita, Fusao
e-Rad_Researcher
00333633
著者名
Sone, Teruo
言語
英語
キーワード
Gene-for-gene
Pyricularia
RAPD
発行日
2009-05
出版者
Blackwell Publishing
収録物名
Molecular Plant Pathology
巻(号)
10(3)
ページ
361-374
抄録
In order to clone and analyse the avirulence gene AVR-Pia from Japanese field isolates of Magnaporthe oryzae, a mutant of the M. oryzae strain Ina168 was isolated. This mutant, which was named Ina168m95-1, gained virulence towards the rice cultivar Aichi-asahi, which contains the resistance gene Pia. A DNA fragment (named PM01) that was deleted in the mutant and that co-segregated with avirulence towards Aichi-asahi was isolated. Three cosmid clones that included the regions that flanked PM01 were isolated from a genomic DNA library. One of these clones (46F3) complemented the mutant phenotype, which indicated clearly that this clone contained the avirulence gene AVR-Pia. Clone 46F3 contained insertions of transposable elements. The 46F3 insert was divided into fragments I-VI, and these were cloned individually into a hygromycin-resistant vector for the transformation of the mutant Ina168m95-1. An inoculation assay of the transformants revealed that fragment V (3.5 kb) contained AVR-Pia. By deletion analysis of fragment V, AVR-Pia was localized to an 1199-bp DNA fragment, which included a 255-bp open reading frame with weak homology to a bacterial cytochrome-c-like protein. Restriction fragment length polymorphism analysis of this region revealed that this DNA sequence co-segregated with the AVR-Pia locus in a genetic map that was constructed using Chinese isolates.
資源タイプ
学術雑誌論文
出版タイプ
Accepted Manuscript
権利情報
The definitive version is available at www.blackwell-synergy.com
PISSN
1464-6722
EISSN
1364-3703
NCID
AA10681726
関連情報
PMID
19400839
関連情報 (isVersionOf)
出版社版DOI
https://doi.org/10.1111/j.1364-3703.2009.00534.x