open access

https://hdl.handle.net/2115/43819

Modification of KDM-2 with Culture-spent Medium for Isolation of Renibacterium salmoninarum

英語

colonization

2009-09

Japanese Society of Fish Pathology

魚病研究

44(3)

139-144

KDM-2 is a medium widely used for isolation of Renibacterium salmoninarum (R.s.), the causative agent of bacterial kidney disease (BKD). KDM-2 still has a problem for colonization of R.s. at low concentration levels. In the present study, we modified KDM by supplementation of the culture-spent medium of R.s. (SMRs) in substitution of FBS. No difference was observed in the growth rate of R.s. at ≥ 10^[3] cells/mL in KDM broth with 1% FBS regardless of SMRs supplementation. Growth rate of R.s. decreased at 10^[1] cells/mL of inoculation into KDM with 1% FBS, but it was recovered by supplementation of ≥ 1% SMRs into the medium. The activity of SMRs supporting bacterial growth was stable to treatment at 60℃ for 30 min and freezing at -20℃ for 7 days. At inoculation of ≤ 300 CFU of R.s., expected colony counts were obtained on the agar plates containing SMRs, while non or less than half of bacteria colonized on the agar plates without SMRs. It was thus considered that the modified KDM by supplementation of SMRs instead of FBS was convenient and inexpensive for isolation of R.s., especially at low concentration levels.

学術雑誌論文

Version of Record

0388-788X