Vascular Cell-Like Potential of Undifferentiated Ligament Fibroblasts to Construct Vascular Cell-Specific Marker-Positive Blood Vessel Structures in a PI3K Activation-Dependent Manner

Okubo, Naoto; Ishisaki, Akira; Iizuka, Tadashi; Tamura, Masato; Kitagawa, Yoshimasa

Vascular Cell-Like Potential of Undifferentiated Ligament Fibroblasts to Construct Vascular Cell-Specific Marker-Positive Blood Vessel Structures in a PI3K Activation-Dependent Manner

Okubo, Naoto; Ishisaki, Akira; Iizuka, Tadashi et al.

Journal of Vascular Research, 2010, 47(5), 369-383

Permalink : https://hdl.handle.net/2115/43855

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アクセス権	open access
URI	https://hdl.handle.net/2115/43855
タイトル	Vascular Cell-Like Potential of Undifferentiated Ligament Fibroblasts to Construct Vascular Cell-Specific Marker-Positive Blood Vessel Structures in a PI3K Activation-Dependent Manner
著者	Okubo, Naoto ; Ishisaki, Akira ; Iizuka, Tadashi ; Tamura, Masato ; Kitagawa, Yoshimasa
著者名 Okubo, Naoto
著者名 Ishisaki, Akira
著者名 Iizuka, Tadashi
著者名 Tamura, Masato
著者名 Kitagawa, Yoshimasa
言語	英語
キーワード	Three-dimensional vessel reconstruction
	Angiogenesis
	Tie-2
	FLK-1
	Differentiation
	Fibroblasts
	Endothelial cell
	Fibroblast growth factor
	PI3K
	Periodontal ligament
発行日	2010-08
出版者	Karger
収録物名	Journal of Vascular Research
巻(号)	47(5)
ページ	369-383
抄録	Objective: To evaluate whether fibroblasts derived from periodontal ligament (PDL) retain the ability to differentiate into putative vascular cells and construct vascular cell-specific marker-positive blood vessel structures. We also evaluated the morphological features of the structure and investigated the intracellular molecular mechanism underlying the angiogenic activity of these cells. Methods: Single cell-derived cultures (SCDCs) were established from primary rat ligament fibroblast cultures, and their expression of ligament cell-, mesenchymal stem cell (MSC)-, and vascular cell-specific markers was evaluated by RT-PCR and immunocytochemistry. The ability of the cells to construct a blood vessel structure was evaluated in a three-dimensional type I collagen scaffold. The morphological and immunohistological characteristics of the structure were then evaluated. Results: Each SCDC expressed endothelial cell (EC) and smooth muscle cell-specific markers, in addition to MSC- and ligament cell-specific markers. SCDC2 cells, which abundantly expressed the EC markers Flk-1 and Tie-2, vigorously constructed a blood vessel structure in a phosphoinositide 3-kinase activation-dependent manner. Conclusion: PDL fibroblasts have the potential to construct an EC marker-positive blood vessel-like structure. Consequently, the fibroblastic lineage in ligament tissue could be a candidate precursor for construction of a vascular system around damaged ligament tissue to facilitate its regeneration.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Copyright © 2010 S. Karger AG, Basel
PISSN	1018-1172
関連情報	PMID 20110728
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1159/000277724