A role of the Ca2+ binding site of DC-SIGN in the phagocytosis of E. coli

Iyori, Mitsuhiro; Ohtani, Makoto; Hasebe, Akira et al.

Biochemical and biophysical research communications, 2008, 377（２）, 367-372

Permalink : https://hdl.handle.net/2115/43954

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アクセス権	open access
URI	https://hdl.handle.net/2115/43954
タイトル	A role of the Ca2+ binding site of DC-SIGN in the phagocytosis of E. coli
著者	Iyori, Mitsuhiro ; Ohtani, Makoto ; Hasebe, Akira ; Totsuka, Yasunori ; Shibata, Ken-ichiro
著者名 Iyori, Mitsuhiro
著者名 Ohtani, Makoto
著者名 Hasebe, Akira
著者名 Totsuka, Yasunori
e-Rad_Researcher 50145265 著者名 Shibata, Ken-ichiro
言語	英語
発行日	2008-10-10
出版者	Academic Press
収録物名	Biochemical and biophysical research communications
巻(号)	377（２）
ページ	367-372
抄録	HEK293 cells stably expressing DC-SIGN (293/DC-SIGN) were examined for phagocytosis of Escherichia coli. 293/DC-SIGN stable transfectants were able to mediate phagocytosis of E. coli. The phagocytosis was inhibited by EDTA or several inhibitors specific for Syk kinase, Raf kinase and the transcription factor NF-􀀁B.DC-SIGN consists of characteristic domains and motifs such as CRD, neck, incomplete ITAM, dileucine and tri-acidic cluster. HEK293 cells expressing mutants of DC-SIGN were also examined for the phagocytosis. It was found that Ca2+ binding sites in the CRD of DC-SIGN were involved in phagocytosis of bacteria as well as multimerization of DC-SIGN, and the neck region played a role in efficiency of binding to microbes as well as multimerization of the protein.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0006-291X
EISSN	1090-2104
NCID	AA00564395
関連情報	PMID 18848918