open access

https://hdl.handle.net/2115/43973

Resveratrol modulates phagocytosis of bacteria through an NF-κB-dependent gene program

英語

2007-02-22

American Society for Microbiology

American Society for Microbiology

52(2)

121-127

Many studies have shown that the pharmacological effects of resveratrol, a phytoalexin polyphenolic compound, include protective effects against cancer and inflammation as well as enhancement of stress resistance. In this study, we examined whether resveratrol affected phagocytosis of bacteria by macrophages and activation of the transcription factor NF-κB after stimulation with or without the ligand FSL-1 for Toll-like receptor 2 (TLR2). Both phagocytosis of E. coli or S. aureus by THP-1 cells or RAW264.7 cells was inhibited by resveratrol in a dose-dependent manner regardless of stimulation with FSL-1. The NF-κB activity in HEK293 cells stably expressing TLR2 was also inhibited by resveratrol after stimulation with FSL-1. Resveratrol also inhibited both the translocation of p65 of NF-κB into nuclei in the transfectant and TNF-α production by THP-1 cells or RAW264.7 cells. It has recently been reported that TLR-mediated signaling pathways lead to upregulation of mRNAs of phagocytic receptors, including scavenger receptors and C-type lectin receptors.This study also demonstrated that FSL-1 induced upregulation of mRNAs of phagocytic receptors such as macrophage scavenger receptor-1, CD36, DC-SIGN and Dectin-1, and the FSL-1-induced upregulation of their mRNAs was inhibited by resveratrol. In addition, it was found that the expression of DC-SIGN in HEK293 cells stably expressing DC-SIGN was reduced by resveratrol and the phagocytic activity was significantly inhibited by resveratrol. Thus, this study suggested that resveratrol inhibited the bacterial phagocytosis by macrophages by downregulating the expression of phagocytic receptors and the NF-κB activity.

学術雑誌論文

Accepted Manuscript

0066-4804

1098-6596

AA00542574