open access

https://hdl.handle.net/2115/43974

The diacylated lipopeptide FSL-1 induces TLR2-mediated Th2 responses

英語

lipopeptide

2006-10

55

FEMS immunology and medical microbiology

48（１）

44-55

44

The diacylated lipopeptide FSL-1 enhanced the generation of IgG antibodies in TLR2+/+ mice, but not in TLR2-/- mice, when administered together with hen egg lysozyme as an antigen. Escherichia coli lipopolysaccharide enhanced the generation of antigen-specific antibodies in both TLR2-/- and TLR2+/+ mice. In TLR2+/+ mice, the enhancement level by FSL-1 was similar to that by lipopolysaccharide. Analysis of the IgG antibodies subclass demonstrated that level of Th2-type IgG1 antibodies was higher than that of Th1-type IgG2a antibodies. Both FSL-1 and lipopolysaccharide induced production of IL-10 and IL-6 by splenocytes from TLR2+/+ mice. Lipopolysaccharide also induced production of these cytokines by splenocytes from TLR2-/- mice, but FSL-1 did not. Neither FSL-1 nor lipopolysaccharide induced IL-12p70 production by splenocytes from both types of mice. FSL-1 upregulated B7.2 expression in B220+ cells from TLR2+/+ mice but not those from TLR2-/- mice, whereas lipopolysaccharide upregulated B7.2 expression in B220+ cells from both types of mice. FSL-1 and, to a lesser extent, lipopolysaccharide activated mitogen-activated protein kinases in splenocytes. FSL-1 and, to a lesser extent,lipopolysaccharide induced the expression of c-Fos, which is known to be involved in Th2-type responses, in splenocytes. Thus, this study demonstrated that FSL-1 possessed TLR2-mediated Th2-type responses in vivo.

学術雑誌論文

Accepted Manuscript

0928-8244

1574-695X

AA10879949