open access

https://hdl.handle.net/2115/43983

Synergic effects of mycoplasmal lipopeptides and extracellular ATP on activation of macrophages

英語

2002-07

American Society For Microbiology

Infection and immunity

70(7)

3586-3591

Mycoplasmal lipopeptides, S-(2,3-bispalmitoyloxypropyl)-CGDPKHSPKSF and S-(2,3-bispalmitoyloxypropyl)-CGNNDESNISFKEK activated a monocytic cell line,THP-1 cells, to produce tumor necrosis factor-α. The activity of the lipopeptides was augmented by ATP added in a dose-dependent manner. In addition, the level of the expression of mRNAs of tumor necrosis factor-α, interleukin-1β, -6 and -8 was also up-regulated by the lipopeptides and/or extracellular ATP, but that of interleukin-10 was not. The P2X purinergic receptor antagonists pyridoxal phosphate 6-azophenyl 2’,4’-disulfonic acid and periodate oxidized ATP suppressed the activity of ATP to augment the activation of THP-1 cells by the lipopeptides, suggesting that P2X receptors play important roles in the activity of ATP. The nuclear factor-κB inhibitor dexamethasone also suppressed the activity,suggesting that the activity of ATP is dependent upon the nuclear factor-κB.Thus, these results suggest that the interaction of extracellular ATP with the P2X receptors is attributed to the activity of ATP to augment the activation of THP-1 cells by mycoplasmal lipopeptides.

学術雑誌論文

Accepted Manuscript

0019-9567

1098-5522

AA00673732