Apoptosis of odontoclasts under physiological root resorption of human deciduous teeth

Domon, Takanori; Taniguchi, Yumi; Inoue, Kiichiro et al.

Cell and Tissue Research, 2008, 331(2), 423-433

Permalink : https://hdl.handle.net/2115/43991

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アクセス権
open access
URI
https://hdl.handle.net/2115/43991
タイトル
Apoptosis of odontoclasts under physiological root resorption of human deciduous teeth
著者
Domon, Takanori ; Taniguchi, Yumi ; Inoue, Kiichiro ; Ushijima, Natsumi ; Taishi, Yoshihito ; Hiramatsu, Akiko ; Wakita, Minoru ; Yoshida, Shigemitsu
e-Rad_Researcher
50217618
著者名
Domon, Takanori
著者名
Taniguchi, Yumi
著者名
Inoue, Kiichiro
著者名
Ushijima, Natsumi
著者名
Taishi, Yoshihito
著者名
Hiramatsu, Akiko
著者名
Wakita, Minoru
著者名
Yoshida, Shigemitsu
言語
英語
キーワード
Odontoclast
Apoptosis
TUNEL method
Transmission electron microscopy
Human
発行日
2008
出版者
Springer
収録物名
Cell and Tissue Research
巻(号)
331(2)
ページ
423-433
抄録
This study was designed to establish the apoptosis of odontoclasts during physiological root resorption of human deciduous teeth. Deciduous teeth were fixed, decalcified, and embedded in paraffin for immunohistochemical (IHC) observations and in Epon for transmission electron microscopy (TEM). Apoptotic cells were identified by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-digoxigenin nick-end labeling (TUNEL), and then tartrate-resistant acid phosphatase (TRAP) activity was determined on the same sections. Epon-embedded specimens were sectioned serially into 0.5-μm semithin sections; some of these sections were re-embedded in Epon, sectioned into 0.1-μm ultrathin sections, and observed by TEM. IHC revealed that the nuclei of TRAP-positive odontoclasts on the dentine were generally TUNEL-negative. Around these odontoclasts, a few TRAP-positive structures were present together with TUNEL-positive structures, e.g., a TRAPpositive structure with one TUNEL-positive nucleus, a TRAP-positive structure with one TUNEL-positive nucleus plus one or two TUNEL-negative nuclei, or a TRAP-positive structure with no nucleus. By TEM, some odontoclasts showed nuclear fragments including compacted chromatin. The results suggest that, during apoptosis, odontoclasts fragment into variously sized cellular parts including three or fewer nuclei.
資源タイプ
学術雑誌論文
出版タイプ
Accepted Manuscript
権利情報
The original publication is available at www.springerlink.com
関連情報
PMID
18000684
関連情報 (isVersionOf)
出版社版DOI
https://doi.org/10.1007/s00441-007-0525-0