Adsorption of egg albumin onto methylated yeast biomass

Seki, Hideshi; Suzuki, Akira; Maruyama, Hideo

Journal of Colloid and Interface Science, 2004, 270(2), 304-308

Permalink : https://hdl.handle.net/2115/44071

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アクセス権
open access
URI
https://hdl.handle.net/2115/44071
タイトル
Adsorption of egg albumin onto methylated yeast biomass
著者
Seki, Hideshi ; Suzuki, Akira ; Maruyama, Hideo
e-Rad_Researcher
10179327
著者名
Seki, Hideshi
著者名
Suzuki, Akira
e-Rad_Researcher
20241375
著者名
Maruyama, Hideo
言語
英語
キーワード
methylation
yeast biomass
adsorption
protein
egg albumin
発行日
2004-02-15
出版者
Elsevier
収録物名
Journal of Colloid and Interface Science
巻(号)
270(2)
ページ
304-308
抄録
A new biosorbent, methylated yeast (MeYE), was prepared for the adsorptive separation of proteins from aqueous solutions. Yeast was methylated in a 0.1 M HCl methyl alcohol solution at room temperature. About 80% of the carboxylic groups of yeast could be methylated within 9 h. The adsorption of egg albumin to MeYE was studied to evaluate the protein adsorption ability of MeYE. At near neutral pH, egg albumin was scarcely adsorbed to unmethylated yeast and the adsorption amount of egg albumin increased with increasing the methylation degree. The adsorption amount of egg albumin to MeYE increased with increasing pH from 4 to 7 and steeply decreased above pH 7. The Langmuir isotherm was applied to determine the apparent adsorption constant and the saturated adsorption amount of egg albumin to MeYE. Both the apparent adsorption constant and the saturated adsorption amount increased with the degree of methylation. The saturated adsorption amount of egg albumin to MeYE having methylation degree 77% was 8.41×10-6 mol g-1 or 0.378 g g-1 at near neutral pH.
資源タイプ
学術雑誌論文
出版タイプ
Accepted Manuscript
PISSN
0021-9797
NCID
AA00695804
関連情報 (isVersionOf)
出版社版DOI
https://doi.org/10.1016/j.jcis.2003.09.039